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By Maurício Guimarães Bergerman, Eduardo Henrique Marsola Niño de Guzmán

A abrasividade de um mineral ou rocha é um tema de extrema importância na indústria mineral e é medido geralmente pelo índice de abrasividade de Bond (AI). Entretanto o ensaio de Bond restringe as amostras tanto pela granulometria utilizada no ensaio, entre ½” e ¾”, como quanto pela massa, sendo necessários 1600 g, além do tempo de realização de aproximadamente 60 minutos. Visando o estudo de abrasividade de materiais mais finos, foi pensado no desenvolvimento de um ensaio LCPC modificado, utilizando granulometrias extremamente finas, da ordem de 1,0 mm ou menores, para previsão de desgaste em circuitos de beneficiamento mineral, antecipando assim possíveis falhas em função da mudança das características de abrasividade do material que está sendo processado, podendo ser utilizado para caracterizar materiais finos da etapa de prospecção, perfuração, pré-concentração, e fluxos da flotação..

Mar 1, 2019

By Éder Adolfo Serafim

A indústria automotiva tem aumentado a utilização de aços de alta resistência, em função dos novos requisitos do mercado, onde deseja-se aumentar a segurança veicular e reduzir a poluição. Os dual phase têm sido apresentados como uma boa alternativa para indústria automotiva, especialmente, em função da alta resistência mecânica e boa ductilidade, ambas oriundas da microestrutura, geralmente, ferrita e martensita. Neste trabalho estudou-se o efeito da adição de crescente de molibdênio em um aço dual phase na formação de austenita na região do campo bifásico. As amostras foram tratadas em diferentes temperaturas no campo bifásico e campo austenítico. Foi utilizado um forno com banho de sal, a fim de aquecer e mantê-las na temperatura durante 60 segundos e, em seguida, resfriá-las em água (têmpera). Com o aumento da concentração de molibdênio até 0,15% obtém-se maior fração volumétrica de austenita, entretanto após 0,15% há uma redução, porém, ocorre refinamento da austenita que atua como mecanismo de aumento de resistência, quando transformada em martensita pelo processo de têmpera.

Aug 16, 2017

By David Guerrero

El presente documento busca exponer la relevancia de la búsqueda de la predictibilidad en la gestión social en el marco del desarrollo de operaciones mineras. Para la búsqueda de la predictibilidad se propone gestionar adecuadamente el conocimiento que se genera producto de los diversos procesos sociales que se emprenden como parte de la gestión social. Como parte de la gestión del conocimiento se proponen herramientas metodológicas que permitan generar aprendizajes y asimismo se logre construir escenarios predictivos ante contextos sociales de similar condición.

Sep 29, 2023

By Guilherme Lisboa Quaresma

Um ensaio por partículas magnéticas é um método não destrutivo utilizado na detecção de descontinuidades superficiais e subsuperficiais em produtos laminados. Este método consiste em submeter a peça a um campo magnético, com aglomeração de partículas magnetizáveis nos pontos onde há descontinuidade. As partículas usadas podem ser de diferentes cores, dimensões e formas. A partícula fluorescente sob a luz ultravioleta demonstra melhor resultado para detecção de pequenas descontinuidades. Para ampliar o campo de visão ensaiado e realçar o contraste entre partícula e peça a radiação ultravioleta deve garantir uniformidade, intensidade e comprimento de onda adequado. Essas exigências motivaram o trabalho da Gerdau junto com a Metal Chek, representante da Spectroline, no desenvolvimento de uma solução inovadora. Na Linha de Inspeção de Tarugos da Gerdau Ouro Branco o uso de luminárias ultravioletas de LED - Light Emitting Diode tornou-se uma alternativa que proporcionou ganho em eficiência e em confiabilidade na tomada de decisão do inspetor de qualidade, baixo consumo de energia, custo de manutenção e satisfação dos clientes. A implantação das luminárias de LED refletiu na prática a evolução da qualidade do produto, além de melhores condições em meio ambiente, saúde e segurança para o inspetor.

Jul 30, 2018

By E J. Coyle, L A. Millington

Tailings storage facilities are geotechnically complicated structures that develop heterogeneous strength profiles and varied porewater conditions. The monitoring of porewater pressures is one of the only direct measurements that can be performed to monitor the performance of a facility throughout its life and is of particular importance in upstream facilities. Foundation underdrainage, continuous and staged tailings deposition, climate inputs (rain, snow, humidity, evaporation, etc), and stratified tailings permeabilities can complicate this critical measurement that introduces uncertainty to stability analyses. These difficult conditions manifest in vibrating wire piezometer measurements as very low or negative pressure readings, both of which are within the instrument’s range of accuracy. However, the data can be difficult to interpret, and a range of interpretations are adopted from optimistic low phreatic surfaces to conservative fully saturated tailings. Where tailings are potentially liquefiable, these very low readings introduce further uncertainty regarding the pore space saturation ratio and liquefaction potential above the estimated phreatic surface. This area of uncertainty is the vadose zone, and its affect on tailings behaviour is not well understood. The two facilities analysed in this paper are upstream, raised embankments with tailings classified as clayey, sandy silt that have been found to be potentially liquefiable. The vibrating wire piezometers (VWP) at both sites show very low or negative readings when they are initially installed near the surface and then go through cycles of re-saturation and de-saturation. Eventually the readings settle as the VWPs are buried deeper within the tailings. The instrumented sections within the tailings column do not show consistent readings that would be indicative of a hydrostatic phreatic surface at either site. Rather, they are highly variable and can be interpreted as either representing a high phreatic surface within the tailings or as a ‘perched’ phreatic zone within the tailings layers. How the VWP data is interpreted has a significant impact on how the facilities are raised and operated. The design outcome for Site 1, which adopts a lower phreatic surface, results in an embankment that meets factor of safety (FoS) requirements under residual strength conditions without any buttressing. Conversely, a conservative (high) phreatic surface has been adopted for Site 2 and has resulted in the facility requiring a significant buttress to meet FoS under residual strength conditions.

Jul 1, 2021

By M Akop, G Phillips, L Elliott

An estimation geologist has many tools in their kit and using the correct one for the job is vital for creating a useful model. Reconciliation is a key step to monitor the performance of a resource model and to ensure the correct tools have been selected and appropriate parameters are set. This paper presents an example of refining the estimation methodology for a Channel Iron Deposit (CID), resulting in an improved model. Rio Tinto Iron Ore (RTIO) CIDs are unique iron ore deposits located in the Pilbara region of Western Australia and contribute a significant volume to the world’s seaborne iron ore market. A problematic feature of these deposits is the irregular dispersion of clay pods that cannot be delineated with confidence within the resolution of the exploration drilling. Resource to grade control reconciliation showed an over-prediction of high-grade material in the resource model. A fresh approach was required to improve grade and tonnage predictions. Historically, clay pods were delineated using traditional wireframing techniques and estimated using ordinary kriging with hard boundaries. The new method utilised indicator kriging to estimate the probability of clay. Probabilities generated from the indicator kriging estimate were used as an additional weighting following the ordinary kriging of grades. Results showed indicator kriging improved global high-grade tonnes reconciliation, however, as expected, it did not improve the spatial prediction of clay. Based on improved reconciliation results indicator kriging has now been adopted as a suitable alternative for analogous CIDs at RTIO. These improvements benefit the mine planning process, but do not replace grade control models.

Mar 22, 2022

By Paul C. Schmall, Kevin Clarke, Paul J. Lewis, John M. Stanbury

"The $200M rehabilitation of the more than 100-year-old Gowanus Flushing Tunnel and pumping station located on the Gowanus Canal in Brooklyn, NY required deep excavations in an extremely congested urban environment. The environment posed significant challenges, including: support of excavation for protection of existing structures, excavation through man-made fill while eliminating groundwater inflow due to potentially contaminated groundwater chemistry; discharge and disposal impacts; and potential settlement of surrounding structures. This paper will present the design and construction challenges associated with the construction of a new combined sewer overflow (CSO) chamberwhich included a jet grout bottom seal, to mitigate groundwater inflow during excavation. The foundation for the finished structure also included a unique system incorporating the jet grout bottom seal elements with deep jet grout columns (piles) acting as the foundation system for the final structure. Design and construction challenges included highly variable soil conditions such as man-made fill, obstructions from previous construction, presence of an organic stratum, and conflicts with existing timber foundation piles. The jet grout bottom seal was constructed in loose sands with the deeper jet grout foundation elements extending to bear on a dense sand stratum. Challenges associated with the bottom seal included grid spacing and construction sequencing. Wet in wet sequencing was used to mitigate potential windows in the bottom seal further complicated by shadowing from existing timber foundation piles.Introduction And BackgroundModernization of the Gowanus Tunnel Pumping System required deep excavations in the extremely congested urban environment of Brooklyn, NY. The project location is shown on Fig. 1 and 2. The focus of this paper is to present the design and construction challenges associated with the support of excavation, control of groundwater, and ultimate foundation support for the combined sewer overflow (CSO) bypass chamber extension. The project site was tightly constrained by adjacent, active roadways and underground utilities, as well as residential and industrial structures. Therefore, excavation support was required to limit ground movement and maintain the integrity of the surrounding facilities. Excavation depths to as much as 16 ft below ground level, 8 ft below groundwater, required that seepage and groundwater be controlled so as to minimize pumping and disposal of groundwater and maintain the surrounding groundwater levels to avoid potential settlement of adjacent roadways, utilities, and structures. In addition, the subsurface conditions required that the proposed CSO bypass chamber extension be supported on a deep foundation to avoid differential and total settlements that may be detrimental to the existing and expanded structure. A plan view of the existing CSO bypass chamber and the proposed extension is shown on Fig. 3."

Jan 1, 2016

By Jonathan G. Cannon

"This paper will describe dynamic pile testing conducted during installation of driven octagonal precast prestressed concrete piles used as the foundation for this bridge. The piles were long and hence spliced. A number of the piles showed damage near the splices and the damage location was near the ground surface so that the damage could be verified using visual inspection. The piles and splice configuration are still in use today without modification. Further WEAP driveability analysis has been conducted which demonstrates the effect of the splice on pile stresses and a likely failure mode. A slight modification is proposed to the splice that should prevent the damage on future projects.INTRODUCTIONThe bridge site is located on the Olympic Way, Route 41, about 20km north of the regional centre of Albury, NSW. For a long distance north of the site the Olympic Way ""shadows"" the main Melbourne to Sydney rail line and is located on the Western side and in close proximity. At the bridge site there was previously a sharp dog-leg in the road and a right angle level crossing with the road changing to the Eastern side of the rail line, eventually joining up with the Hume Highway, which in turn now becomes a ring-road through the eastern outskirts of Albury. The dog-leg and rail crossing had warning signs and bells, speed restrictions and rumble strips to warn approaching motorists to reduce speed. Site distance was exceptionally good.Approximately 2years before construction a group of five ""high spirited"" young men from the local area were driving south on the Olympic Way adjacent to a south-bound train. It is understood they interacted with the train crew for a period of time. Unfortunately the car and train collided at the level crossing and all of the young men were killed. There were outpourings of grief in the local community and a decision was made to replace the level crossing with a road-over-rail bridge.The bridge was highly skewed with two piers and two high level abutments with fill embankments on both approaches.GROUND CONDITIONSThe site is open grazing country with approximately level ground at the site with surrounding low hills. The geology was unremarkable with consistent stiff clays for a considerable depth.The design required driven precast prestressed pile foundations with the piles to be driven to a considerable depth.Hard driving with high tension stresses were evident from the start of driving and a variety of different pre-bore lengths were adopted in an attempt to minimise driving and potential for damage. At the abutments the pile locations were predrilled for at least the full embankment depth.PILESThe piles were 550mm octagonal prestressed concrete with the pier piles being single piece and the longer abutment piles being two-piece with a ""standard"" epoxy glued box splice. Pier piles and the initial length of the abutment piles were 24m. The add-on piece used at the abutments was 12m. Pile concrete was grade 50MPa."

Jan 1, 1900

By Carlos Tarazaga

Progressive Design-Build (PDB) is an emerging variation of alternative delivery programs in the underground construction industry. PDB refers to the way a construction project design is developed by the Owner and the Design-Builder in a step-by-step process. According to the Design-Build Institute of America (DBIA), Progressive Design-Build allows the design and construction team to collaborate during the earliest stages of project development. This enables engagement between the three key players in a construction contract: the owner, the designer, and the contractor. Is the PDB the best alternative program delivery for large contracts? What is the perspective of the private sector? This paper will provide an overview of the different alternative delivery programs used in the underground industry, pros and cons of each of programs, and the perspective of the designers and contractors. The paper will focus on how the PDB works, reasons why owners should consider PDB for their projects, best collaboration strategies among owners, designers, and contractors to deliver a successful project, and how to manage the project risks using a PDB method.

Jun 13, 2023

By Anthony Garotinho Barros Assed Matheus de Oliveirar, Wagner Anacleto Pinheiro, Andreza Menezes Lima

Óxido de grafeno foi sintetizado e posteriormente reduzido pelo uso de vitamina C. Para comparação entre o óxido de grafeno (GO) e o óxido de grafeno reduzido (rGO) foram utilizadas as técnicas de difração de raios X (DRX), microscopia eletrônica de varredura (MEV) e espetroscopia Raman (ER). Na etapa da síntese do óxido de grafeno por DRX confirmou-se a efetiva oxidação do grafite precursor, enquanto por MEV observou-se a esfoliação dos flocos de grafite em folhas de GO. Após a redução, por meio de DRX e ER analisou-se a remoção dos grupos funcionais, enquanto por MEV a morfologia das folhas do material.

Nov 16, 2019

By Luana Lessa Pacheco, Antônio Vitor Magalhães Cota

With the sharp fall in commodity prices, the terms cost reduction and higher productivity have become subjects that are constantly addressed in the daily life of companies, since these factors represent a question of survival of the organizations. To achieve this, the search for continuous improvement of our processes in order to guarantee space in the market is a reality and necessity, so it is important that companies know their processes in detail and have the maturity to make decisions based on data and facts. Thus, this work aims to apply the Six Sigma methodology to investigate which are the variables that most influence the transportation process, carried out by large trucks, increasing their specific consumption of diesel, which is the second largest expense of a company. mining in the city of Mariana MG. The goal of the project was to reduce diesel consumption in the large transport fleet by 7.4%, from 0.32 l / t (average carried out from Feb / 16 to Jan / 17) to 0.30 by February 2018 , with an estimated gain of R $ 4.2 million. The results achieved exceeded the established target, reaching a 10% reduction in specific diesel consumption, totaling a projected real gain of R $ 6.4 Million, in addition to the reduction of 1,710.52 t of CO2 in the atmosphere.

Oct 3, 2019

By Peter Hodnik, Gabriele Paulon, Herbert Moser, Thierry Gautreau, Loris Busolini

At voestalpine Stahl, Linz a new conventional slab caster has been installed to produce mainly automotive and electrical applications grades, implementing the latest design solutions: tight pitch roll geometry, mould and strand stirring and braking devices, SEN water modelling validation, enhanced roll cooling design to allow dry casting conditions, finely defined slab cooling system layout to control slab surface temperature across the width. To control these design solutions the latest generation of technological packages has been implemented like detailed solidification model based on meshless computational algorithm and a fully dynamic application of soft reduction to achieve sound internal quality results.

Oct 1, 2019

By Majid Saghaeian, Rasool Hejazi, Parisa Ghobadi

"Pulp handling in mineral processing circuits is very complicated due to its inherent different specifications compared with other fluids such as water. Because any variation in ore property (e.g. size and abrasion index) and pulp specification (e.g. density, solid content and pressure) causes problem in this part and reduces the plant availability and throughput as a result. The most common issues in the hose system are sumps and pipe-line blockage, wearing of the pumps impeller and liner, and pipe tearing specially at elbows and bends. High pressure grinding roll (HPGR) is employed in a closed circuit with a screen as the first stage of grinding in Zarand Iranian Steel Complex (ZISCO). Wearing of the pump and pipeline transferring the screen undersize stream was the main problem from the plant commissioning. The plant couldn't achieve to the design capacity because of this defect. Although various practical solutions such as improving the pipes and impeller material quality and eliminating the elbows, as much as possible, were done in order to downtime minimizing, but damages didn’t vanish. Since HPGR discharge contains somewhat rough and abrasive particles, the mentioned modifications didn't effective very much. Therefore, converting of the wet screen to dry and using of belt conveyor to the screen undersize handling was employed based on the plant capability evaluation and experimental and pilot trial results. Although this reformation imposed another additional cost (for conveyor system), but it had significant advantages such as increasing the plant availability up to 96%, reducing the operating costs of the wear parts, providing more stable operating condition for the HPGR performance and decreasing abrasion rate of the HPGR rolls liner because of the lower moist feed."

Jan 1, 2014

By S. L. Chen

The Kumera Technology Center developed a unique type of steam dryer for drying copper concentrate in the mid 1990s. The dryer has been chosen in several copper smelter projects since 1999. To expand production capacities, the Norddeutsche Affinerie smelter, the Pirdop smelter of Cumerio, the Toyo smelter of Sumitomo Metal Mining and the Tamano smelter of Hibi Kyodo Smelting have carried out projects in which Kumera Steam Dryers were installed. The capacity of a single steam dryer varies from 70 t/h to 160 t/h. This article summarizes the latest operation results of the Kumera Steam Dryers in these smelters and introduces the progress of the ongoing projects in Yanggu Xiangguang Copper, Guixi smelter of Jiangxi Copper, Jinlong Copper, Boliden Harjavalta, and Onsan smelter of LS-Nikko Copper.

Jan 1, 2007

By Victor Li, Joley Lam

"INTRODUCTIONJacked piles are piles that are pressed into the ground, usually by hydraulic jacks. For this reason, jacked piles are also known as pressed in piles. Jacked piles are mainly used for the following applications.• Underpinning worksJacked piles have been commonly used for strengthening the foundation of existing structures. In pressing the pile into the ground, the hydraulic jacks will jack against existing pile caps or ground beams that support the structure or against a steel frame connected to the floor slabs or structural walls of the structure. The self weight of the structure provides the reaction force for pile jacking. Jacked piles used for underpinning works usually have lower design ultimate capacities, typically less than 2000 kN (225 tons).• New foundationsJacked piles can be used to support new structures. A heavy pile jacking machine is employed to provide the reaction force for jacking the piles into the ground.• Retaining structuresJacked piles can be installed at close spacings to form a retaining wall. For relatively large and easily accessible sites, the piles can be installed using a conventional pile jacking machine. For sites that are smaller and are more difficult to access, a more compact patented equipment called the Silent-Piler developed by Giken Seisakusho Co. Ltd. in Japan can be used.Jacked piles have several distinct advantages. The vibration and noise induced during pile jacking are minimal. Jacked piles are 100 percent proof-tested to a load level equal to the maximum applied jacking force. The pile jacking machine can also be treated as mobile kentledge for static loading test. In this article, discussion will focus on jacked piles used for supporting new structures.HISTORICAL DEVELOPMENTThe People’s Republic of China (PRC) is perhaps one of the earliest countries in which jacked piles were used as new foundations. Jacked piles had been installed in the PRC since the 1960s. The capacities of jacked piling machines used in the country are usually in the range of 3000 to 6000kN (337 to 675 tons). High capacity jacking machines with a capacity of 9000 kN (1000 tons), presently used by the construction industry in Hong Kong, were also manufactured in the PRC. There are also plans to produce machines with a capacity exceeding 10000 kN (1125 tons). It was projected by Wang (1999) that jacked prestressed spun concrete piles would become one of the most popular pile types in the PRC in the 21st century."

Jan 1, 2004

By Daniel Torres Espinoza, Jose Pinedo

"Ventilation is a vital service in underground mines, required to provide fresh air to the work force and dilute diesel equipment gases (CO, NOx) and diesel particulate matters (DPM). At a Mexican precious metal mine in the state of Chihuahua there is a VOD system implemented using Howden’s control and monitoring system and a state of the art tagging and tracking technology. The JM zone of the mine is ventilated using a push and pull system, using automated regulators on each level to control the air going in and out of the levels. The implementation of advanced control strategies using Howden’s VentSim™CONTROL allows the mine to generate significant savings and increase health and safety of the workers.INTRODUCTIONIn underground mines, ventilation is a requirement in order to maintain the fresh air needed for personnel and equipment. Ventilation regulations dictate the amount of air required underground to carry out day to day operations by limiting the exposure that workers have to toxic gases and other pollutants. Regulations for required airflow in underground operations for diesel pollutants are generally provided on vehicle rated brake horsepower, in Mexico the requirement is 75 cfm/bhp. In underground mines, ventilation services can account to up to 75% of the mine’s energy consumption, and it is very common for fans to operate all the time because of a lack of control infrastructure for the ventilation system. By the implementation of a Ventilation On Demand (VOD) system a mine can reduce this consumption with different control strategies. The main infrastructure required to implement this system consists of remote control of ventilation infrastructure (fans and regulators), a communication network between throughout the mine with the surface and a tracking system for personnel and diesel equipment. Software is required to determine fresh air requirements based on the tracking system data and then send the control commands to the different ventilation infrastructure. In 2015, San Julian Mine acquired Howden’s VentSim™CONTROL (formally SmartEXEC) and the hardware solution to deploy a VOD system at their operation. The VOD system and Fresnillo plc’s San Julian Mine in Chihuahua, Mexico, consists of control of 10 surface exhaust fans, 2 surface intake fans, and 12 underground automated regulators. It also includes the monitoring of 46 flow sensors, 28 CO sensors, 28 NO?? sensors and 42 temperature and humidity sensors, approximately 500 personnel tags, approximately 220 vehicle tracking tags with diesel status (ON or OFF) and approximately 250 tracking zones. This paper discusses the control strategies that have been implemented at San Julian Mine since the system was commissioned and the next steps that will be taken at the mine with the system as they test different level on VOD."

Jan 1, 2019

By D. J. MacKinnon

Zinc metal was first produced in India around 1100 A.O. and a zinc industry based on retorting gradually developed in both India and China(1) . The vertical retort method for smelting zinc ores was developed in England in the 1730s; this was followed by the more fuel efficient horizontal retort process developed by the Belgians in the 19th century(2) . The horizontal retort process was the standard method of zinc manufacture until the 1950s when it became obvious that it could not compete with electrolytic zinc technology(2). The present day extractive metallurgy of zinc is dominated by the electrolytic process which now accounts for almost 80% of the world's primary zinc production. In recent years the electrolytic zinc process has seen significant improvements: the fluid-bed roasting of zinc concentrates, improved leaching and residue treatment techniques such as the jarosite and goethite processes, improved solution purification procedures, mechanization of the zinc electrolysis step and of the zinc melting and casting operation. In addition, new leaching processes have been implemented for the treatment of oxidized ores, particularly silicates, and the pressure leaching of zinc sulphide concentrates is now a commercial reality. Although the electrolytic zinc process was first used on a commercial scale in 1916, its history can be traced back to the 1860s. The purpose of the present paper is to outline the early history of the electrolytic zinc process and to trace the significant developments which led to the first successful commercial electrolytic zinc plants.

Jan 1, 1999

By David White

When finished (projected completion in 2016) the Hong Kong-Zhuhai-Macau Bridge Project will comprise three cable-stayed span bridges and a 4.15 mile immersed tube tunnel - linking Hong Kong to Macau and the mainland Chinese city of Zhuhai. The design included two artificial Islands to serve as the transition structures for the tunnel to bridges, entailing a massive seaborne land reclamation project, and the use of thousands of sheet piles, and man-hours. The challenges of driving individual steel sheet in 100? of water are well known, let alone in the open waters of the South China Sea. In 2010 a joint effort between American Piledriving Equipment (APE) and Allnamics Pile Testing Experts convinced the Chinese contractor First Harbor Marine Group China that a massive multi-vibro hammer can be used to drive 130? long, 72? diameter steel pipe piles weighing 662 US tons each into the sea bed of the South China Sea for the Hong Kong - Zhuhai - Macau bridge artificial islands. Combining APE?s hammer design, production and operation know-how with the pile driving simulation and installation know-how of Allnamics made it possible to show the feasibility of driving these gigantic piles into bottom of the sea to the required depth.

By William Conger, Greg Schick

Grinding media consumption is a major cost variable in industrial mineral comminution circuits. Specifically, industrial SAG media consumption results from a combination of surface abrasion wear, media breakage, surface spalling and rejection size from the mill. Three main consumption rates; weight of media consumed per time, per kWh and per weight of ore ground are defined and compared. Recent industrial data has provided an opportunity to relate consumption in terms of weight of media consumed to weight of ore ground (g/tonne) and weight of media consumed per kWh of energy consumed (g/kWh) with SAG specific grinding energy consumed (kWh/tonne). Using relationships developed from this data it is possible to project SAG media consumption in future mineral comminution circuits.

Jan 1, 2014

By Eugene V. Cilento, R. Clark Lantz, K. A. DiGregorio

"This laboratory bas developed a sensitive electro-optical method to quantify the initial rate (R) and total amount (MAX) of superoxide (02) produced by single pulmonary alveolar macrophages (PAM). The method uses a microscope-based TV system to visualize PAM in culture, and to video record the images during the time the cells produce 02. MAX and R are calculated from measurement of temporal changes in optical density in the images due to precipitated diformazan formed by the reaction of O2-, produced by each PAM, with nitroblue tetrazolium present in the culture medium. To date, values of Rand MAX, measured due to adherence of PAM to the dish, have been compared to values obtained when quartz, coal mine dust (CMD), and kaolin . were added to the medium (in vitro). Rand MAX have also been calculated for PAM lavaged from animals exposed to quartz and CMD in the WVU Inhalation Facility. Presently, experiments are being done using which will permit PAM to be restirnulated by different dusts to help establish a dose response relation~ p and a means to study the role of lung surfactant on modifying the acute toxicity of inbaled dusts. Ultimately, this methodology should provide useful insight into establishing the role of 02 in PAM dysfunction due to inhalation of dusts, and in lung diseases such as pneumoconioses. INTRODOCTIONPAM are free cells found in the lungs which protect the lungs by removing foreign debris and bacteria. This is accomplished, in part, by the process of phagocytosis, whereby foreign matter is internalized into vesicles known as primary phagosomes. Phagocytosis also involves the chemical breakdown of inhaled dusts and the killing of microbes. Detoxification is aided by the respiratory burst, which is a metabolic response of the cell to foreign substances, that results in the production of highly reactive oxidizing agents from the partial reduction of extracellular oxygen to superoxide (02) at the surface of the plasma membrane. Superoxide undergoes either spontaneous or enzyme catalyzed dismutation reactions to form hydrogen peroxide (H202) and subsequently hydroxyl radical (OH•) and singlet oxygen (02 t ). However, while these oxygen metabolites aid in the killing of microbes they also may destroy endogenous tissue. For example, 02 has been linked to the aging process and to many diseases including emphysema, diabetes, and cancer.'' Therefore, a better understanding of the production of 02 is extremely important since an abnonnally low production could result in damage by inhaled dusts and bacteria while an abnormally high production could result in direct damage to the lung tissue by the phagocytes. Inhalation of respirable sized mineral dusts, such as quartz (silica), coal mine dusts (CMD), and asbestos, results in various pulmonary disorders. PAM are thought to play an important role since evidence suggests that the first step in fibrogenesis is an interaction of dust particles with PAM. Generally, in vivo exposure of animals to mineral dusts results in increased respiratory burst activity, migratory patterns, phagocytic behavior, and secretory potential of PAM, and for this reason these have been implicated to be pivotal events in the pathogenesis of pulmonary diseases.1 In addition to stimulation of resident PAM, an influx of monocytes into the lung and/or production of new macrophages generally occurs in response to fibrogenic dust inhalation. Further, PAM may attract and stimulate fibroblasts, which normally synthesize proteins and collagen during rep~ of tissue, by secreting chemoattractants and enzymes. Thus, PAM may be involved in pulmonary disorders through failure or partial loss of their defensive capability, or indirectly, through release of other mediators."

Jan 1, 1990