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By W. G. Pariseau

"BACKGROUND Strata mechanics of three underground coal mines in central Utah were recently studied using a specialized finite element technique [1, 2, 3, 4]. The purpose of these studies was to examine relationships between mining and related seismicity. The longwall mining method was predominant, but room and pillar mining and barrier pillar mining were also used in the mines studied. In every case, high correlation between face advance and seismicity was observed. The specialized finite element technique allows for whole mine subsidence estimation at a kilometer scale and for stress concentration at a meter scale near a working face. Comparisons of observed mine subsidence with model subsidence allows for model verification and justifies detailed analysis of the evolution of stress at seam level. Yielding is expected near a longwall panel face and as the face is advanced the extent of yielding is expected to increase. The total number of element failures in a finite element mine model also increases, so a high correlation with associated seismicity, say, event count, is not so surprising. A brief review of recent coal mine studies in Utah is presented in the sequel. The procedures are given in some detail in application to trona mining. COAL MINES The analysis procedure begins with the geologic column and proceeds to specification of strata properties including elastic moduli and strengths. Joints are an integral part of the geologic model and joint set specifications include dip direction, dip, spacing, stiffness and strength. Spatial variability of strata properties in situ may be taken into account during analysis. Topography is important, especially to Utah coal mines that are developed from outcrop in regions of high relief. Mesh generation follows specification of data input. Finite element analysis is next, followed by reporting of results. In this regard, all finite element computations are based on first principles. No model “calibration” is done that involves adjusting computer input to obtain a fit to mine measurements such as subsidence profiles. Figure 1 illustrates the concept of a dual node – dual mesh finite element concept. Dual nodes are roof and floor nodes in mined areas. These nodes replace the seam. The dual mesh is a much refined mesh with displacement boundary conditions obtained from the big mesh and thus replaces the effect of mining in the region outside the dual mesh. The refined dual mesh allows for details of stress, strain and displacement near a working face of interest. Strata Properties Strata properties include elastic moduli and strengths of intact rock between joints and joint stiffness and strength for each joint set present. Both properties sets may be obtained from laboratory test data, although testing joints poses a sampling problem in the field. The statistical variability observed in laboratory test data, say, in unconfined compressive strength tests is often characterized by much variation. In fact, the coefficient of variation, ratio of standard deviation to mean, is often greater than 35 percent. When sample values are mapped back to the mine location, one may observe spatial variability as properties vary from place to place as shown in Figure 2. Spatial variability may be incorporated into a finite element analysis with probabilistic assignment of properties to elements in accordance with measured variability. The result is a more realistic model of rock in situ."

Jan 1, 2017

By Reno Pressacco

Abstract- Agrium Inc. is a world leader in the production, distribution, and marketing of agricultural products and services in both North America and Argentina. The new phosphate mine and mill complex located southwest of Kapuskasing, Ontario, was constructed to replace an offshore supply of phosphate rock, and produces a high-grade phosphate concentrate. The Cargill Township Carbonatite Complex is one of a series of carbonatite complexes in the region that are hosted by the 1.9 Ga aged Kapuskasing Structural Zone. The carbonatites in the mine area have intruded Archean gneissic granodiorite rocks of the Wawa Subprovince, and metasedimentary rocks of the Quetico Subprovince. The Cargill Carbonatite Complex displays a typical concentric zonation pattern from an outer ring of alkalic pyroxenites, through an inner ring of sövite-carbonatite, to a central core of rauhaugite-carbonatite. The initial carbonatite intrusion and subsequent residual orebody have been modified by faulting such that the northwestern third of the complex has been displaced approximately 2.5 km northeast. The phosphate ores are composed principally of residual apatite sands that were formed by the weathering and dissolution of the soluble minerals of the carbonatite protolith. These ores are subdivided by their iron content into high grade (A ores) and lower grade (B1 and B2) ores. They are separated from the primary unweathered carbonatite by a layer of saprolite, whose thickness varies depending upon the composition of the underlying carbonatite. Cretaceous waste materials consisting of well-sorted silica/kaolinite sand and peat deposits are typically found to stratigraphically overlie the ores. A minor amount of the ore feed comes from cemented phosphate ores. All rock types are covered by Pleistocene glacial tills and clays.

Jan 1, 2001

The visit which took place on the 18th February, 1972 was a departure in that the places visited had no direct connection with the work in which members of the Institute are normally engaged. It was refreshing and stimulating to see important and involved operations normally outside ones own trend of life and to have experts to speak about technical, financial and matters of general interest concerning their work, not forgetting their troubles and problems and how they overcame them. Carlton Centre The members of the Institute were received by Mr D. Appleton, Public Relations Officer of the Carlton Centre Limited, who introduced Mr L. R. M. Fee of W. Rhodes Harrison, Architect in charge of the project; Mr W. C. E. Skilton of Murray and Roberts, the Engineer in charge of the construction of the complex and Mr A. Home the Senior Operating Engineer of Carlton Centre Limited. Each of these gentlemen addressed the members giving short rundowns on the project with special reference to their particular sphere of the undertaking. A variety of particularly interesting statistics relating to the planning construction and the ultimate operation and maintenance of the complex was given. Mining engineers present had an opportunity to compare the problems encountered by them associated with depth to problems encountered by the civil engineer in the construction of a building rising to such an enormous height. To some of the members a sense of similarity of the labour problems encountered must have been recognised. After tea the visitors were divided into groups, each under the guidance of an official, and then visited the highest floor accessible and thereafter the 19th floor where the internal arrangements for airconditioning, lighting, plumbing, power supply and communication arrangements were explained. Thereafter a visit to one of the seven basements which houses all machinery required to serve this enormous complex enabled the visitors to see the standby power arrangements as well as the refrigeration plant. The visit finished in time to proceed to the Crest Hotel in Hillbrow for lunch. To our hosts for the visit to the Carlton Complex a special vote of thanks is recorded. Strydom Tower In the afternoon the party proceeded to the Strydom Tower where Mr Knight from the Public Works Department who was the Engineer in charge of the construction of the tower gave details on the construction and the extraordinary methods which had to be adopted to achieve the desired objectives and to overcome known as well as unforeseen difficulties. The comparatively short time taken to complete the project and the good safety record appeared to impress the visitors. The final phase of the visit was taken care of by Mr Bothma of Telecommunications Department of the Post Office, who gave an extremely interesting explanation of the telecommunication organization of the Post Office with particular reference to the part the Hillbrow Tower plays in the South African telecommunication system. Both gentlemen answered many questions willingly and interestingly. Following the explanatory talks the visitors were shown the technical equipment housed in the two top levels of the tower. Short explanatory talks gave members an insight into the workings of one of the most important aspects of modern life. Even to the amateur photographer opportunities existed to view and photograph the city from the highest available observation point. We are indebted to Mr Gouws, the Regional Director of the Post Office for granting permission to visit sections of the tower normally not accessible to the general public and to our hosts for the manner in which we were received and entertained on the explanation of the many interesting features of this important installation. In conclusion, the programme for the day can be classified as interesting, of exceptional educational value, and an opportunity to see something of the engineering skills put into two vast and ambitious undertakings and the results achieved.

By Zetti Gavelán

Globalization and the search for new ore deposits inevitably lead to investment in foreign markets. Investors need to be aware of the effect of their venture on regional, social and economic issues when they invest. Decades of detailed statistics from various sources can be integrated and analyzed to predict the social-economic impact of investment, thereby mitigating risk. To this end, the correlation among variables such as foreign direct investment into mining (FDIM), reserves, and socio-economic indexes is examined. The investigation focuses on South America from 1992 to 2000. Throughout the past decade, South America has benefited from capital investment from more developed countries. This huge investment by the mining industry has impacted the South American economies and can be measured directly using the FDIM. Other economic variables investigated include mining gross domestic product, direct employment, and human development indexes. The correlation is based on data compiled from the World Bank, international risk assessment agencies, the United States Geological Survey (USGS), and the information agencies of various South American countries. Initial analysis of the economic data suggests various expected outcomes. For example, foreign investors may disregard a measured, highly negative country risk in deference to the quality of its mineral resources. It is important to note that the standard measures of country risk published by investment analysts are not industry or investor-specific. In the case of the investor, the level of accepted risk is based on their managed investment portfolio. It is noted that arguments for level-playing fields may be the antithesis of globalization. The discovery and exploitation of ore bodies in a globalized environment necessitates the development of economic measures that standardize mining-specific risk variables and trends. The latest published information on the security of mineral investments in South America is investigated by integrating databases of economic, geologic, production, human indexes, and risk ranking.

May 1, 2003

By Ellsworth Y. Dougherty, Edwards F. Fitzhugh

"INTRODUCTION During the summer of 1945 the Bureau of Mines made approximately 200 miles of reconnaissance magnetic surveys in north-central Mirnesota. The survey started near the western end of the Mesabi Iron Range and extended into the area to the southwest, west, and northwest and also southward to the-northern limits of the Cuyuna Iron Range. The purpose of the survey was to data to assist exploration for iron ore in the region through:1. Supplying a relatively unit form network of traverses, by which and future surveys of localities in the urea ray be correlated. Suggesting localities in which more detailed magnetic surveys may be justified, and, Disclosing localities in which there is lass hope of obtaining significant results from further magnetic surveys.The instrument used was a Hotchkiss Superdip, and traverses for calibration and comparison were run with a Schmidt-type magnetometer. Field observations and work directly related thereto were carried out under contract by E. J. Lang-year Co., Minneapolis. Engineers of the Bureau of Mines planned, directed, and reviewed the survey."

Aug 1, 1946

By E. A. Sondreal

The Bureau of Mines investigated the use of lignite char for producing carbon disulfide; CS2 production rates were as high as when wood charcoal was used as raw material. The maximum space velocity for CS2 produced in the bench-scale study was 9,280 hour-1, reacting sulfur and lignite char at 792° C. The reaction temperature and the temperature at which the char was carbonized were the two most important factors in determining CS2 production rates. Increasing the carbonization temperature in nitrogen from 400° to 900° C advanced the temperature at which carbon disulfide was first produced from 550° to 850° C. The average activation energy for reacting chars with sulfur was 44,000 cal/g mole. The reaction was first order with respect to the partial pressure of sulfur. Increasing the rate of sulfur admission caused a decrease in rate of production at otherwise similar conditions. Rates were not affected by either char size or sodium carbonate catalysis. High space velocities at moderate temperatures using inexpensive lignite chars could offer an economic advantage in the manufacture of carbon disulfide provided that yields were attractive.

Jan 1, 1967

By W. A. Young

Bull Lake is one of several saline lakes situated a few miles north of Rawlins, Carbon County, Wyo. (figs. and 2).. The Bureau of Mines conducted preliminary investigations of sodium sulfate deposits in many of the lakes and performed detailed work on Bull Lake. This investigation consisted of drilling 11 vertical holes through salt beds using diamond core bits and Iwan-type auger bits. Bull Lake lies in a shallow, closed depression in loosely consolidated sand. The salt beds, ranging in thickness from 2.5 to 26 feet, occur above a thick bed of shale of upper Cretaceous age. The salts appear to have been deposited in the lake from surface and subsurface drainage. The source of salt is undoubtedly the enclosing sedimentary rock's exposed to the west of the basin. Sodium sulfate in the deposits probably is present as mirabilite or Glauber salt (Na2SO4-10H2O). Chief impurities in the deposits arc mud and sand. Other soluble salts present average approximately 1 percent combined. Cores from the 11 holes were analyzed in the Bureau of Mines laboratories at Salt Lake City. Drilling totaled 512 feet. Results of analyses are given in the appendix.

Jan 1, 1951

By H. Dong, J. J. Valencia, L. Nastac

"A comprehensive computer model was developed to understand and optimize the centrifugal casting process for manufacturing TiC/Al-bronze friction drums. Performance of these components requires proper distribution of TiC particles, which is shown by dimensional analysis to be dominated by centrifugal buoyancy effects and solidification kinetics.The model addresses the following: (1) a rheology-viscosity model of the interference between moving particles, (2) engulfment (entrapment) or pushing of particles in the mushy region by the advancing solid/liquid interface, (3) nucleation and growth of equiaxed and eutectic phases, (4) effects of particle size, particle concentration, and cooling rate on the final grain size, and (5) the impingement effect of particles on the nucleation and growth kinetics of solidifying microstructure.Parametric studies were conducted using the computer model to investigate the effects of various process and material parameters on the distribution of TiC particles. The effects of the volume fraction, size, and morphology of the particles (including clusters and agglomerates) on the particle distribution were evaluated in detail. The complex interaction between the solidifying structure and insoluble ceramic particles in centrifugally-cast metal-matrix-composites was also investigated.The model predictions of particle distribution and microstructure were validated with experimental data for centrifugally-cast A356/SiC and TiC/Al-bronze alloys.This work was conducted by the National Center for Excellence in Metalworking Technology, operated by Concurrent Technologies Corporation under contract No. N00140-92- C-BC49 to the U.S. Navy as part of the U.S. Navy Manufacturing Technology Program."

Jan 1, 2000

By D. M. Aloi

In today's evolving and globalized world, mining companies are being pressured to move beyond compliance towards long-term sustainability and sustainable development. Companies are expected to "engage" with communities and measure the success of these engagements without a clear understanding of "how" to do this. This move, away from "Corporate" Social Responsibility (CSR) and compliance, develops more of a focus on "Community" Social Responsibility and sustainability. This shift in focus leads to the development of world-class projects that provide sustainable development for people, reduces the incidence of costly delays, and promotes good global citizenship for corporations. The process of "engaging" communities in mining project design and development leads to a stronger license to operate and sustainable development. To create this mutually beneficial relationship between communities and companies, communities must be "engaged" in the mining process as early as possible, preferably during the exploration or the concept phases. How to create this "engagement" remains elusive. How to measure the progress of this engagement is also problematic. The quantitative and qualitative metrics needed to measure both positive and negative impacts of the mining and community relationship will be discussed.

Jan 1, 2007

Project-finance risk assessment is mature and approached systematically. Mining industry due diligence remains fragmented with specialists addressing different aspects of the project such as reserves/JORC, environmental impact, commodity market, even financial analysis/ modelling. Each professional has a natural tendency to believe that the risk factors they have examined are the key ones necessary to be reviewed, as if their review will stand in isolation from others in the way a project materialises. By first laying out the project risk categories and risk matrices, this paper examines the due diligence process with a keen eye to the risk assessment and overlap/interaction. Some cross fertilisation of due diligence techniques from other industry sectors is placed into the mining context. Six case studies are then presented with regards to due diligence features in each projectÆs feasibility review or financial information/ offering memorandum. A power case first shows the differences between the due diligence expectations of banks versus bond financings. Then some Australian and Indonesian mining development cases are analysed to see the due diligence learning points. Some of these were project/due diligence failures. The conclusion focuses on the value of a systematic risk assessment for project evaluation, all from the project-finance perspective.

Jan 1, 2007

By Jesús L. Valenzuela, José R. Parga, Salvador Aguayo, Héctor Moreno

The Merrill Crowe zinc-precipitation process has been applied worldwide on a large number of operations for the recovery of gold and silver resulting in a higher consumption of zinc dust from cyanide solutions. However, in some of plants this precipitate is of low quality, because lead, cadmium and especially copper are precipitated along with gold and silver. The low quality precipitate also requires more fluxes in smelting and shortens crucible life. In this study, a process is introduced to remove copper, silver, lead and cadmium from barren solutions following the Merrill-Crowe process. Copper and silver are removed by precipitation in a serpentine reactor, using sodium sulfide as the precipitator, and sulfuric acid for pH control. The recovery of lead and cadmium ions with natural hydroxyapatite using cow bone powder (CBP) as a new adsorbent material, is introduced. CBP is capable of removing lead and cadmium ions from aqueous cyanide solutions with 99% efficiency. It has the ability to compete with other existing commercial adsorbents for the removal of lead and cadmium. Results show that pH value has a significant effect on copper cyanide removal efficiency, and it was determined the optimal pH range to be 2 - 3. At this pH value, the copper cyanide removal efficiency achieved was up to 99 %; and for the cyanide associated with copper cyanide complexes, the recovery was 90%. Finally, the precipitate quality was improved 400% .This procedure was successfully run at three mines in México.

Jan 1, 2014

By Robert Hopler

THE work that is being done by the Government to reduce the dangers of mining was brought out strikingly in the First National Mine-Safety Demonstration which occurred at Pittsburgh, Pa., on October 30th and 31st, under the auspices of the United States Bureau of Mines with the assistance of the American National Red Cross Society and the Pittsburgh Coal Operators’ Association.

Jan 1, 2012

By James L. Elder

The Bureau of Mines and the Alabama. Power Co. jointly conducted a first experiment in underground gasification of coal at Gorgas, Ala., during the fall and winter of 1946-47. This preliminary experiment showed that it was not difficult to maintain combustion of coal underground, and that coal in place could be completely gasified. It was noted that the high temperature developed by the gasification of coal in place brought about changes in overlying strata that appeared to be favorable to the process. The high temperatures caused the roof rock to become plastic, to expand, and to settle down on the mine floor directly behind the reacting coke face. These results were promising enough to warrant further investigation of the process. It was believed that if this action could be controlled properly with roofs of similar composition, it would tend to force the gas-making fluids underground against the coal faces. Plans for the second experiment were based in part on this favor-able roof action, and the successful operation of the planned initial straightline passages depended on this fact. Gorgas was chosen as the site for the second experiment for the following reasons: A tract of coal land of sufficient size was available and isolated from the main body of coal; the roof rock was similar in composition to that encountered in the first experiment; and the overlying strata were thick enough to eliminate some of the operating difficulties that had been encountered, In addition, power, water, and machine-shop facilities as well as an adequate supply of labor were available in this area, and the friendly cooperation of the Alabama Power Co. further recommended this site for the planned experiment.

Jan 1, 1951

By William Seymour

"INTRODUCTION Byproduct-coke manufacture is one of our country's most essential in¬dustries; its importance was strikingly accentuated by the demands of World War II. This paper reviews recent progress in various phases of byproduct-coke operations for the production of metallurgical coke particularly that used as blast-furnace fuel.In the early days of the by-product-coke industry in the United States high-grade bituminous coking coals, both high-volatile or gas coal and low-volatile Pocahontas coal, were plentiful, and the selection and mixing of coals for coke-oven use were minor problems.The demands of World War I for the production of iron, steel, and coke aroused and stimulated new interest in the investigation of the reserves, characteristics, and properties of our coking coals. During that war the increased demand for coke reached a critical stage, and the shortage of high-grade coking coals necessitated the use of cols of inferior quality and of different coking characteristics than those previously used.The period of prosperity following World War I brought much progress and development in the by-product-coke industry. Methods of testing coal and coke were improved. The search for satisfactory coking coals was continued, and construction and design of byproduct ovens were improved."

Jun 1, 1946

By E. C. Foster

The project objective of this contract is to design, develop, and demonstrate a dust collecting system for auger head miners, used in coal seams 40 inches or less in height, which will reduce respirable dust below the limits established by the Federal Coal Mine Health and Safety Act of 1969. This system was to be incorporated into 3 Wilcox Mark 20 continuous miner. In order to accomplish this, the objective was divided in three main areas. First: To develop a dust control system which would remove by isolation, respirable dust from the face area and areas where miners work. Second: To develop a dust scrubbing system to remove and collect float dust before the air reaches the return airway. Third: To reduce the overall noise levels to which the miners are exposed.

Jan 1, 1974

By Mengmeng Gai

This paper introduces a model-based automatic object recognition and registration framework to assist heavy equipment operators in rapidly perceiving 3D working environment at dynamic construction sites. A video camera and a laser scanner were utilized in this study to rapidly recognize and register dynamic target objects in a 3D space by dynamically separating target object?s point cloud data from a background scene for a quick computing process. A smart scan data updating method has been developed which only updates the dynamic target object?s point cloud data while keeping the previously scanned static work environments. Extracted target areas containing 3D point clouds were orthographically projected into a series of 2D planes with a rotation center located in the target?s vertical-middle line. Prepared 2D templates were compared to these 2D planes by extracting SURF features. Point cloud bundles of the target were recognized, and followed by the prepared CAD model?s registration to the templates. The field experimental results show that the proposed rapid workspace modeling method can significantly improve heavy construction equipment operations and automated equipment control by rapidly modeling dynamic target objects in a 3D view.

Aug 1, 2013

On 8th August, 1973, 57 members of the Institute visited Prieska Copper Mine at Copperton in the North Western Cape. After tea, the group was welcomed by the Management of the Mine, and Mr Grobler, the General Manager, gave an introductory talk. After they had been taken on a bus tour of the property, the members split up into groups and proceeded underground or to the concentrator. Of special interest to the mining members were the rock-handling methods in use at the mine. The stoping method that has been adopted is the continuous, long-hole sub-level open stoping system. The broken ore is moved with rubbertyred front-end loaders from the stope drawpoints to the ore passes. On the main tramming levels, the ore is collected by electric locomotives and discharged into the main shaft system at the bottom. At the shaft ore-pass system, a gyratory crusher that can accept rocks up to 1 m in diameter has been installed. The crushed product (minus 150 mm in diameter) will then be hoisted by a Koepe winder, which has a rated capacity of 900 tons per hour. The mine is also served by a declined roadway from surface, which facilitates the movement of heavy equipment. The most striking aspect is the productivity that can be achieved by the use of a trackless-mining system. For the production of 230 000 tons per month, it is estimated that 6 stopers and 7 developers will be required. The total labour force will be approximately 2500-2000 Bantu, 200 Coloured, and 300 Europeans. Training of maintenance and operating personnel is of the utmost importance, and it was interesting to note the extent to which non-White labour is being used in these occupations. Lunch was served at the single quarters, after which the group proceeded to the airfield for the return trip to Johannesburg. On behalf of the visitors, Professor de V. Lambrechts thanked Mr Grobler and his staff for the most interesting morning and for their hospitality,

Jan 10, 1973

By John W. Buch

Safety and cost in mining coal beds under the extreme pressures found at depth, full extraction to conserve resources, and even surface subsidence to minimize damage of improved areas have directed much well-deserved attention, in the United Kingdom; to roof-control methods. Because the effects of pressure will be felt intensely as our Pennsylvania anthracite mines grow deeper, the senior author took advantage of an opportunity afforded by a mission to Europe3/ to observe, to a limited extent, the effects of pressures found in deep mines and the manner in which they are con trolled. Organized research relating to strata control has been carried on in Great Britain for nearly 25 years. These activities have been ably outlined by Dr. D. W. Phillips.4/ Physical properties of coal-measure rocks, movements of roof and floor, and types of face and roadway supports have been investigated. Same of the forces induced by the extraction of coal have been measured. The results of this research have been publicized and openly discussed, and much progress has been made in establishing a better factual understanding of roof-control problems and their solution.

Jan 1, 1951

By P. M. Mashaba, S. O. Bada

An advanced high internal phase water-in-oil (HIP W/O) emulsion binder and flotation with an internal dispersed water phase of 95 vol% was used to beneficiate South African weathered coal from discard dumps. Five coal samples with calorific values ranged from 8.48 MJ/kg to 20.94 MJ/kg were utilized. The performance of the HIP W/O emulsion was compared to that of kerosene in terms of the physicochemical properties of the clean coal products. A 5 kg/t emulsion binder addition produced a high-quality coal concentrate with 11.76% ash and a heating value of 28.61 MJ/kg from a sample containing 29.34% ash. Using the same dose and coal sample, kerosene resulted in a clean coal concentrate with 17.47% ash and a calorific value of 26.42 MJ/kg. Further samples were also beneficiated using 5 kg/t of W/O emulsion. The highest increase in calorific value was achieved from the beneficiation of a sample containing 48.71% ash, from 8.48 MJ/kg to 18.16 MJ/kg. It has been established that emulsion binders can upgrade coal samples dumped many decades ago.

Aug 2, 2022

By M. W. Barger, J. K. H. Ma, J. Y. C. Ma, V. Castronova

The objective of this study was to investigate the effects of various bisbenzylisoquinoline (BBIQ) alkaloids on respiratory burst activity of alveolar macrophages and to characterize the interaction of these drugs with alveolar phagocytes. BBIQ alkaloids were chosen for study because they exhibit a wide range of antifibrotic potencies in a rat model, with tetrandrine being very effective and tubocurarine being ineffective. These drugs inhibited zymosan-stimulated oxygen consumption with a potency sequence of tetrandrine (IT) = fangchinoline (FA) > berbamine (BE) ., cepharanthine (CE) "" cycleanine (CY} ~ tubocurarine (TU). This inhibition of respiratory burst activity could not be attributed to a drug-induced decline in the ATP content of these pneumocytes. Drug binding to alveolar macrophages was directly dependent on temperature and drug concentration. The sequence for binding capacity was FA > TT = BE = CY > CE ~ TU. Therefore, there was no simple relationship between binding capacity and inhibitory potency. Binding capacity was not related to lipophilicity of these alkaloids. In addition, tetrandrine failed to bind to metabolically dead cells or sonicated macrophage preparations. These data suggest that the interaction of BBIQ alkaloids with phagocytes is not simply nonspecific binding to membrane lipids. Alteration of the cytoskeletal system with vinblastine, taxol, or cytochalasin B decreased tetrandrine binding by approximately 33% when adtkd separately and by 93% when added jointly. Pre-exposure of alveolar macrophages to stimulants increased the ability of BBIQ alkaloids to inhibit both oxygen consumption and superoxide release. These data suggest that the mechanism by which BBIQ alkaloids inhibit activation of phagocytes involves microtubules and bules and microfilaments. Pre-exposure of macrophages to stimulants would change the conformation of cytoskeletal components and may make these structures more susceptible to drug interaction.

Mar 1, 1992