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By Michele Ashby

The mining industry is facing new risks from peak commodities, the energy and economic crisis and stock market pressures. The Green Mining Industry Initiative is a risk management strategy for the future of the mining industry. Defining the Risks The bad news is peak commodities, energy and economic crisis and stock markets collapsing are the issues that are already hitting everyone?s bottom line and draining the treasuries of every mining company around the world. Higher energy costs are affecting the bottom line more than ever before, which is creating more risk for companies. Mining companies have major land positions around the world and they have major energy needs. Uncontrollable increases in energy costs, increased regulations for diesel fuel emissions and energy intermittencies are costing mining companies more time, money, and man-power, which are cutting into profits and hitting the balance sheet. The good news is, there are practical energy solutions which are off the grid, tried and true methods which can be implemented in a cost-effective manner and will increase profitability and save shareholder values as well as energy security for these companies and their projects. To date, most of the mining resource industry has not yet considered or used other forms of power like clean, sustainable, or alternative energy for their own energy needs. The reasons have primarily been pushback from the mining industry due to beliefs of older technologies in alternative energy, a lack of understanding of the cost advantages long term, and perceptions of environmental relativity.

Jan 1, 2008

Gekko Systems Pty Ltd have developed the Python Underground Processing Plant for the concentration of gold and sulfide ores. The Python plant has an established flow sheet including fine crushing, screening, gravity separation and flotation which aims to concentrate the valuable minerals (gold/sulfides). The aim of the plant is to achieve high valuable recovery into a mass yield of 10 - 30 per cent to reduce the amount of material that is needed to be taken to downstream processing. This paper examines the test work protocol for testing an ore to assess its suitability for the Python flow sheet. This paper will discuss the test methods employed to determine crushing requirements, continuous gravity recovery and flotation performance and how the results relate to Python design for the comminution, gravity and flotation circuits. Results from past test work will be discussed.

Aug 1, 2010

By M. Ramokhothoane, S. Rupprecht

"NTRODUCTION Women employment in the mining industry dates back to the 19th century. Various parts of the world, for example, India, Japan, the United Kingdom and the United States of America had women employed in large numbers. The women’s jobs were primarily manual in nature. Women were used to carry coal in buckets from the coal face and out the mine, and those who worked on the surface plants were used to sort waste rock from the coal product. Other heavy industries, which were traditionally male dominated, for example the military, also had a considerable number of women supporting the war effort. The outbreak of war also caused a shortage of labour, which in turn, necessitated that women were used in various roles during the war. After World War II, women returned to their traditional roles, but had acquired new skills, and had a different outlook to life. The wearing of pants and short hair became fashionable, as a result of being in the army.Various laws were passed which prevented the employment of women underground. Different countries had rules that they believed were in the best interest of women and were of the opinion that the rules somehow were protecting women against the harsh working conditions. In South Africa, post 1994, there has been a shift in thinking in regard to the employment of women in the mines. WOMEN IN SOUTH AFRICA MINING In the words of the Minister of Women in the Presidency, Minister Susan Shabangu, “women must take centre [sic] stage as the country gears itself for the implementation of radical socio-economic transformation policies and programmes in the next five years” (SA Government News Agency, 2014). In as much as some progress has been made in advancing women, more needs to be done in order to realize sustainable benefits in the economy. The emancipation of women is a reality and remains top of the South African government agenda. From the Authors’ experience there are two types of men in the mines. The first group support change and see the value in it. To them, women in mining bring in the diversity and play the complementary role in achieving greatness. There is no fear of job security and humiliation but a great sense of “camaraderie”. The other group continues to enforce stereotypes, which works against the spirit of redressing the imbalances of the past."

Jan 1, 2018

By D. Lauriski

The recent spate of catastrophic events at U.S. mines raises the question as to whether or not the mine safety and health system in the U.S. is broken. The prescriptive regulatory regime in the U.S. is not a cure-all for a broken system. Without totally dismissing the contribution of a prescriptive regulatory approach, this paper argues that safety in the U.S. mining industry would be enhanced if the nation adopted a scheme that provides a balance between specification standards and two alternatives: performance and systems-based standards. This would allow effective and flexible means to continuous health and safety performance improvement. Mine operators, regulators and politicians could then go beyond thinking that compliance alone equals strong safety by creating a proactive system that emphasizes continuous improvement. By adopting performance and systems-based standards, exemplary safety and health performers can be recognized, allowing regulators to focus their efforts on those without sound occupational safety systems and who have substandard safety performance. Mining Engineering, 2011, Vol. 63, No. 7, pp. 77-82. Official publication of the Society for Mining, Metallurgy and Exploration, Inc.

Jan 1, 2011

By J S. J van Deventer

"Conventional wisdom is that gold not assayable is not recoverable or does not exist. There has been no formal study on the possibility that gold assays using advanced spectroscopy such as mass spectrometry and neutron activation could grossly underestimate the gold recoverable by proprietary extraction techniques. Such gold is not considered here to be æinvisibleÆ to detection but rather æabnormalÆ or ædistortedÆ at atomic level. Large-scale extraction of gold from æabnormalÆ ores has not been demonstrated, hence this emerging field still lacks credibility and acceptance. For fear of harming their reputation, few academic researchers or companies are expected to venture into this field until it is well proven. This paper aims to create awareness amongst metallurgists of the potential of æabnormalÆ gold ores by reviewing unconventional observations, the pseudoscience mainly available in non-peer reviewed web sites, and hypotheses for the recovery of ænon-assayableÆ gold, including: the occurrence of high levels of nano-sized gold in clays detectable by high-precision analytical instruments, which represents æinvisibleÆ gold accounts of ambient transmutation of elements, mainly using thermal methods orbitally rearranged monoatomic elements (ORMEs), which are virtually undetectable by conventional means, and their conversion to normal metals the possibility of a æhigh spinÆ state of transition metals the formation of microclusters altering the chemical behaviour of gold and the possibility of clustering with other elements. It is suggested here that gold in ores displays a range of clustering, from ænormalÆ gold amenable to conventional extraction to the ORME state. The concepts of æintermolecular waterÆ in forming complex salts locking up precious metals, electro-dissolution in strong acids, and electrowinning at both the anode and cathode are discussed as a means of treating æabnormalÆ gold ores. It is also possible that the gas phase extraction of gold chlorides under controlled temperature and pressure conditions, as well as transferred-arc plasma fuming of gold through carbothermic reduction of sulfides, could be used as a basis for extraction of gold from æabnormalÆ ores.CITATION:van Deventer, J S J, 2013. Is there gold that we do not recognise?, in Proceedings World Gold 2013, pp 27-34 (The Australasian Institute of Mining and Metallurgy: Melbourne)."

Sep 26, 2013

By P Malkin, S Thomas, P M. Stone, D Kostyuk

In open pit mining, it is common that the bottleneck to productivity and profit lies in the truck-shovel mining operation, especially as strip ratios and mining intensity have increased with resource depletion and advances in mining equipment capabilities. This paper outlines a study of truck-shovel productivity using a new proprietary discrete event simulation platform for truck-shovel operations called MineDES. The study will specifically address the issue of whether truck queuing at shovels should be considered productive or not, and how mining engineers can determine the optimal truck fleet size for specific operational configurations.The MineDES simulation platform will be described and illustrated, highlighting the features that make it an appropriate tool for conducting the experiments at the heart of this study. The study encompasses a set of experiments, consisting of a number of simple truck-shovel operational scenarios for a benchmark open pit mining operation, which are simulated using MineDES. Each experiment is designed to highlight different factors, which are most important in calculating the ideal truck fleet size and for understanding the marginal productive impact of each additional truck added to the fleet. The scenarios will principally address the issues of how different types and levels of unscheduled equipment downtime and process variation, combined with the traffic delays/congestion arising in more intense mining operations, will affect productivity. The results of the experiments are interpreted using a simple fit-for-purpose cost and revenue model in order to highlight the indicative cost of over- or under-trucked configurations.The study has found that the optimal fleet size that maximises productivity and value will, in the presence of even small process variation, lead to non-zero trucks queuing at shovels on average. As process variations and unscheduled downtime events increase in the truck-shovel system, the required fleet size to optimise productivity and value increases. MineDES provides a convenient platform for quantifying this for real operations.CITATION:Kostyuk, D, Thomas, S, Malkin, P and Stone, P M, 2014. Is truck queuing productive?, in Proceedings Orebody Modelling and Strategic Mine Planning Symposium 2014 , pp 267–274 (The Australasian Institute of Mining and Metallurgy: Melbourne).

Nov 24, 2014

By B D. Burford

High intensity stirred milling using the IsaMill has been the enabling technology for many projects by transforming the economics of fine grinding and simplifying circuit design. IsaMill grinding technology was originally developed for large-scale grinding to P80 sizings of 7 ¦m. The IsaMill is a high throughput, high efficiency mill that produces sharp product size distributions in open circuit configuration. The ability to grind in an inert environment, using a wide variety of media types, can give further metallurgical and economic benefits. The high energy intensity results in a small footprint that enhances the ability to distribute grinding power throughout the circuit, applying grinding energy to the minerals that need it. Recent scale up developments in IsaMill technology have enabled the benefits to be extended to large-scale conventional grind sizes, until now the domain of ball and tower mills. This paper will examine the crossover of IsaMill technology from ultrafine applications into the more conventional grinding area (P80 20 - 150 ¦m). Scale-up developments in IsaMill technology that have enabled this are reviewed. The upcoming commissioning of a 3.0 MW M10,000 IsaMill, the most powerful to date, grinding to P80 55 ¦m at PPL in South Africa will be discussed, as will the recent commissioning of a 2.6 MW M10,000 IsaMill at Kumtor. Recent testwork at McArthur River, where an IsaMill is being considered as an addition to the primary grinding circuit to replace the current tower mill will be reviewed. The potential combination of HPGR and IsaMill to transform concentrator grinding economics will also be discussed. Large-scale IsaMill technology has the ability to transform circuit design and energy efficiency for both coarse and fine grinding.

Jan 1, 2006

Originally developed as a step change in ultrafine grinding efficiency, the IsaMill? has made inroads into conventional ball mill and tower mill mainstream grinding duties during the last 5 years - beginning with the Anglo Platinum mainstream grinding projects in South Africa. At the 2006 SAG Conference, plant testwork on the incorporation of IsaMill? technology into the McArthur River (Northern Territory, Australia) Pb/Zn primary grinding circuit was reported. The IsaMill? has now become an integral component of that circuit, treating up to 1mm top size feed and directly producing P80 45 µm rougher flotation feed. Two 1.1 MW IsaMills? are now used in this duty contributing to a 27% increase in overall plant throughput and a reduction in rougher feed size distribution. In this paper, we report on the current state of development and performance of the IsaMill? in the primary grinding duty at McArthur River and also consider future directions.

Jan 1, 2012

By G S. Anderson, N W. McDonald

"The IsaMill™ was originally developed to address liberation issues at Mount Isa Mines (now Glencore) operations, McArthur River and Mount Isa in the early 1990s. Following on from the industrial success at those operations, the IsaMill™ was commercialised. In the year 2000, Kalgoorlie Consolidated Gold Mines (KCGM) became the first external user of the technology with a 1.1 MW M3000 IsaMill™ installed to supplement the roaster capacity at its Gidji Processing Plant. The IsaMill™ was used to ultra-fine grind about 10 t/h of pyrite flotation concentrate to 10–12 µm prior to gold cyanide leaching. Based on the success of the Gidji installation, another was installed at the processing plant at KCGM’s Fimiston operation in 2002 in a similar duty.In 2014, a 3 MW M10 000 IsaMill™ was selected to replace the roasters at Gidji as part of the A$98 M emissions reduction project. The mill was commissioned in April 2015 at the design 30 t/h, allowing the immediate shutdown of the two roasters. Replacement of roasting with ultra-fine grinding completely eliminated air emissions at Gidji while maintaining overall plant production rates.This paper details the history of the IsaMill™ at KCGM from the original M3000 to the latest M10 000. It covers the basis of the process selection, circuit design, operating and maintenance data.Citation:Anderson, G S and McDonald, N W, 2016. IsaMill™ at Kalgoorlie Consolidated Gold Mines - from the M3000 to the M10 000 and Replacement of the Roasters at Gidji Processing Plant, in Proceedings 13th AusIMM Mill Operators’ Conference 2016, pp 29–38 (The Australasian Institute of Mining and Metallurgy: Melbourne)."

Oct 10, 2016

MIM Holdings Limited has acquired a reputation for the successful application of R&D to develop break-through technologies for the mining industry. The Isa Process tank-house technology has been licensed to copper refineries throughout the world, and almost 40 per cent of the worldÆs copper is refined using this technology. Isasmelt copper and lead smelting technology is either in use, or is currently being installed, by eight companies in eight countries around the world. Jameson Cell technology is widely used in the coal mining industry and increasingly in the base-metal industry. The IsaMills developed at Mount Isa and McArthur River made it possible to develop the McArthur River and George Fisher orebodies. This paper will address the need for innovation and then discuss the development of the IsaMill and Isasmelt technologies. It will conclude with some comments about why MIM has been so successful in developing such technologies.

Jan 1, 2002

By Michael Mann, Kenneth K. Eltschlager

The International Society of Explosives Engineers (ISEE) Standards Committee has recently updated both the Field Practice Guidelines for Blasting Seismographs and the Performance Specifications for Blasting Seismographs. The revisions are the result of public and industry input along with recommendations of the ISEE Blast Vibration and Seismograph Section. The committee is comprised of blasting seismograph manufacturers, users and general interest personnel. On an as needed basis, subject matter experts are enlisted to advise the committee. Three phases of activities are necessary for each revision cycle: request for comment on the existing standard, first draft revision of the standard based on public input, and final draft approval by the ISEE Board of Directors. The ISEE standards revision process will be explained and each standard will be discussed along with the significant revisions from the latest revision cycle.

Jan 1, 2018

By R. W. Davis

INTRODUCTION CHURCHILL river was named for John Churchill, first Duke of Marl-borough and third Governor of the Hudson's Bay Company, in the latter part of the 17th century, some fifty years after the ill-fated Danish expedition, led by Munck, had abandoned the site of the now famous seaport of Churchill, as a worthless, cold, disease-ridden country. No other attempts were made to explore the interior until the river basin became a part of the grant to the Hudson's Bay Company and began to play its part in furnishing the luxury of fine furs to civilized people as it had furnished the necessities of food and dress for untold centuries to uncivilized natives. This was the sole contribution of the region to the economic life of the country for some two hundred and fifty years, until 1926, when the power site at Island Falls was discovered by engineers seeking a cheap power source to make possible the development of the great ore-body at Flin Flon. DRAINAGE BASIN OF CHURCHILL RIVER The river is some 1,325 miles long. Rising in the headwaters of the Beaver river, in east-central Alberta, it flows in an easterly direction across Saskatchewan and northeasterly across Manitoba to Hudson bay, through a remarkable basin of some 115,500 square miles, only 6,000 square miles less than the area of the British Isles. A journey down this river leads one through three distinct belts of country, each vastly different from the others geologically and in surface characteristics. For the first five hundred miles the river flows through a basin of 36,000 square miles of the great central-plains country, which is covered with fertile soil and carpeted in its southern part with prairie grass, which gradually gives way to the mixed forests of poplar, spruce, birch, and jackpine in the northern and eastern sections: About midway across Saskatchewan, bald-headed knolls and ridges begin to make their appearance as the river meets the western margin of the Canadian Shield. Then, for some seven hundred miles, it passes through country underlain by the pre-Cambrian rocks of the Shield, which continue to within one hundred miles of Hudson bay. Here the character of the country again changes to a low, clay plain underlain by sandstone, and through this 'clay belt' the river flows between steep clay banks, to empty into Hudson bay.

Jan 1, 1935

By Andrew Abraham

Continual improvement is the key to success in today?s competitive global arena. You require tools that promote dynamic quality management, deliver cost savings to your bottom line and provide the foundation for process improvement. ISO 9000 and ISO 14001 management systems and OHSAS 18001 are those tools. Since their inception, the benefits of ISO management systems and registration have been touted as the way to achieve better bottom line results. This spurred many companies to embark upon the road to registration with high expectations. The potential benefits of ISO management system implementation and third party registration that are commonly provided by Registrars and consultants are compelling adverts for success. ISO 9001:2000 is promoted as an essential tool for systematic efficient and affective management controls that provide a framework for analyzing errors and improving a company?s internal operational processes. It also allows companies to comply with internal and external customer and regulatory requirements thus continually improving the quality of their products and services and thus enhancing customer satisfaction. This, in turn, leads to greater customer recognition and increased market share. ISO 14001 implementation and registration may assist your company in reducing its litigation risk, raw material and energy consumption, waste disposal costs and possibly non tariff trade barriers. It should also provide continuity of operations, enhanced operational efficiency and consequently a competitive advantage in the market place. Registration of your ISO 14001 management system should also improve your company?s profile and credibility leading to regulatory flexibility and improved community goodwill. All of this will be a catalyst for improved morale and pride by employees and management alike. Likewise, OHSAS 18001, the latest certification specification for Occupational Health and Safety Management Systems supports Occupational Health and Safety legal and regulatory obligations and compliance, manages & controls Occupational Health and Safety risks and improves performance, safety culture and efficiency. OHSAS 18001 is an audit certification, and not a legislative requirement. Suggested benefits of implementation include reduction in accidents, lost time and associated liability and insurance costs and improving reputation and credibility through demonstrated commitment to health and safety. The foregoing increases access to new customers, joint venture partners and financing The skeptics say these are motherhood statements and lack corroborative evidence, i.e., ?Show me the money!?

May 1, 2003

By W. Kempson

"Mining Companies have producing assets in increasingly diverse portions of the world. It is not uncommon that the owner, the design team, the mine contractors and the operators are all based in different countries. While these assets are always subject to the regulatory requirement of those countries there have not been international standards available to review and evaluate these assets. This becomes particularly important when considering safety critical items such as tailings dams, vertical shaft systems and autonomous vehicles to highlight a few. The work currently being completed in ISO ensures these standards will be available and will be generated by and subscribed to on an international basis, thus removing the potential for patriotic partisanship and ensuring safe and predictable systems are installed. This paper will discuss the benefits of the international standards, the structure of the teams and working groups who are generating these standards as well as the process by which the standards are generated. This will allow insight into the creation of the standard and will aim to demonstrate the “neutral” and technically strong manner in which the generation of these standards is achieved. In addition, the paper will discuss the standards which have been produced in the last five years as well as the current standards which are in place. The finale will discuss upcoming work and the timelines in which it is anticipated these will be completed. INTRODUCTION Mining Companies have producing assets in increasingly diverse portions of the world. While these assets are always subject to the regulatory requirement of those countries, there have not been international standards available to review and evaluate these assets. This becomes particularly important when considering safety critical areas such as vertical shaft systems, tailings dams and autonomous vehicles to highlight a couple. The work currently being completed in the International Organization for Standardization (ISO) ensure these standards will be available and will be generated by and subscribed to on an international basis, thus removing the potential for patriotic partisanship and ensuring safe and predictable systems are installed. The ISO/TC 82 – Mining was founded in 1955. This was an active committee until it was thought that the standards at the time were adequate and the Technical Committee was put on “Care and Maintenance”. In 2012 countries lobbied ISO to pull this committee from dormancy and restart it, this happened in in the same year."

Jan 1, 2019

By M D. Sussman

A large number of countries in the world now have some regulation for labelling the presence of genetically modified organisms (GMO) in food. For the last ten years, ISO/TC 34/SC 16 has been providing methods, guidelines and requirements concerning the evaluation of molecular biomarkers including GMOs. This activity makes sure that stakeholders on all sides of commercial transactions are satisfied and their marketing or regulatory needs are met. Developing and maintaining a transparent, science-based environment for users of horizontal methods for molecular biomarker analysis is a high priority. Sampling and analysis of molecular biomarkers has become critically important for food and agricultural organisations developing, producing, distributing and selling genetically engineered, non-genetically engineered, identity preserved and/or organic crops and their derived products and preventing their respective destructive diseases. To aid in international harmonisation, a new work item proposal to develop an ISO type A management system standard (MSS) for organisations including laboratories using molecular biomarker analysis and sampling has been submitted to ISO/TC 34/SC 16. The purpose of this MSS is ensuring that determination of the presence, absence or quantification of a particular biomarker with a relationship to a product quality determinant, a marketing claim, a regulation or a plant pathogen is carried out under a suitable quality assurance / quality control system. This MSS will provide an overarching system to apply and manage molecular biomarker analysis and sampling for any organisation large, medium or small in a private, government or academic setting.CITATION:Sussman, M D, 2017. ISO/TC 34/SC 16 horizontal methods for molecular biomarker analysis – molecular biomarker analysis and sampling management system, in Proceedings Eighth World Conference on Sampling and Blending , pp 295–302 (The Australasian Institute of Mining and Metallurgy: Melbourne).

May 9, 2017

The concept of isokinetic measurement of strength was originally related by Hislop and Perrine.(1)Characteristics of an isokinetic exertion are constant velocity throughout a predetermined range of motion. Strictly speaking, a means of speed control, and not a load in the usual sense, is applied in isokinetic exertions.(1)However, load and resistance are definitely present in this technique. In this case, the load is a result of the energy absorption process performed by the device to keep the exertion speed constant. The device prevents energy from being dissipated through acceleration in isokinetic exercise. The energy is instead converted into a resistive force, which varies in relation to the efficiency of the skeletal muscle. Since the speed of motion is held constant in isokinetic exercise, the resistance experienced during a contraction is equivalent to the force applied throughout the range of motion. For this reason, the technique of isokinetic exercise has sometimes been referred to as accommodating resistance exercise. This type of exercise allows the muscle to contract at its maximum capability a tall points throughout the range of motion. At the extremes of the range of motion of a joint, the muscle has the least mechanical advantage, and the resistance offered by the machine is correspondingly lower. Similarly, as the muscle reaches its optimal mechanical advantage, the resistance of the machine increases proportionally. It must be understood, however, that while isokinetic devices control the speed of the exertion, this does not assure a constant speed of muscle contraction. The speed of isokinetic contractions is constant during individual exertions; however, it is also possible to compare muscular performance over a wide range of isokinetic velocities. Increasing the isokinetic speed of contraction will place increasing demands on Type II muscle fibers (fast twitch and fast oxidative glycolytic). Workplace Assessment It is clear that isometric strength testing cannot substitute for dynamic strength assessment when examining highly dynamic occupational job demands. As most industrial work tasks contain a significant dynamic component, analysis of isokinetic strength capabilities appears to offer some advantage to isometric testing in this regard. However, it must be recognized that isokinetic devices are not entirely realistic compared with free dynamic lifting in which subjects may use rapid acceleration to gain a weight-lifting advantage.

Jan 1, 1998

By K. A. Natarajan, K. Karthiga Devi

"A biodegradable flocculant was produced during growth of Bacillus megaterium. The major component of the bioflocculant was found to be a polysaccharide composed of some proteins. Fourier transform infrared (FTIR) spectra analysis revealed the presence of carboxyl and hydroxyl groups in the bioflocculant, and thermal characterization by differential scanning calorimetry (DSC) showed the transition and crystalline melting point at 90-105°C. The effects of bioflocculant dosage and pH on the flocculation of mineral suspensions were evaluated. The bioflocculant exhibited good flocculating capability on mineral suspensions and achieved flocculating efficiencies of 90 percent for kaolinite and 85 percent for hematite suspensions at a dosage of only 5 mL/L. The maximum arsenite removal was found to be 90 percent at a bioflocculant dosage of 2 g/L, which is better than traditional chemical flocculants. This study demonstrates that microbial bioflocculants have potential for application in environmental cleanup, such as in the flocculation of mineral fines and in the remediation of solutions that contain toxic heavy metals.IntroductionBioflocculants are biopolymers produced by microorganisms during their growths. The predominant components of a bioflocculant are extracellular polymeric substances such as glycoprotein, protein, cellulose and nucleic acid (Salehizadeh and Shojaosadati, 2001). Bioflocculants have gained much wider attention due to their biodegradability and safety. In recent years, the study of bioflocculants has attracted wide attention. Bioflocculants can be nontoxic, harmless and eco-friendly. Normally, they exhibit different functional properties, serving as stabilizer, dispersant or thickener. Among these properties, flocculation is of major interest. For example, Phormidium sp. strain J-1, isolated from a drainage channel, exhibited excellent flocculation activity for bentonite particles in liquid suspension (Fattom and Shilo, 1984). A protein bioflocculant produced by Rhodococcus erythropolis could efficiently flocculate suspended solids while losing their flocculating activity by enzymatic digestion (Kurane et al., 1994, Takeda et al., 1991). Klebsiella sp. strain S11 screened from activated sludge was unable to flocculate without the addition of calcium chloride (Dermlim et al., 1999)."

Jan 1, 2015

By S. R. Rannels, D. E. Rannels

"I. IntroductionThe alveolar surface of the lung is covered by two populations of pneumocytes, type I and type II epithelium. The type I cell, which accounts for most of the alveolar surface area and provides a highly specialized barrier for gas exchange (Rannels et al., 1991), is poorly understood. Although approaches to type I cell purification have been discussed, they have not been applied widely because of the lack of phenotypic markers to monitor isolation procedures (Dobbs et al., 1988; Danto et al., 1992). In contrast, the type II cell has been studied extensively, largely because of the development of reliable procedures for its isolation and primary culture. Investigations based on the use of purified populations of cultured type II cells have provided convincing evidence to support a role for the pneumocytes both in the maintenance of normal lung function and in the response of the lung to injury (for review, see Rannels and Rannels, 1989).While type II cells can be isolated by a variety of approaches, the protocols used most commonly are modifications of the gradient method of Dobbs and Mason (1979) or of the panning method of Dobbs et al. (1986). Purification of the cells based on density gradient centrifugation is reliable and consistent, with yields of 30 to 40 X 106 type II cells per rat of 230 g body weight. Isolation procedures applied to a minimum of three to four rats provide the best results, as lower cell yields are common with the use of fewer animals. The protocol below is designed to obtain a maximal cell yield from the lungs of six rats.II. Materials and InstrumentationMaterialsCalbiochem: DNase I (Cat. No. 260912); Corning: culture flask, 75 cm2 #25115; Costar: culture plate, 24-well #3424; disposable serological pipette #4101; Elastin Products Inc: elastase #E-134; Falcon: cellulose nitrate membrane filter, 0.22 µm, #7105; conical tube, 50 ml, #2070; culture dish, 100 mm, #3003; culture plate, sixwell, #3046; Fisher: BaS04 , #B68-500; NaCl, #5271-3; NaHC03 , #5233-50; Fort Dodge Laboratories: pentobarbital, #1206-lE; Gibco: Dulbecco's minimal essential medium (DMEM), #430-1600EC; Hanks' balanced salt solution (HBSS), #310- 4185AG; Joklik's modified Eagle's medium (JMEM), #410-2300EC; HyClone Laboratories Inc: fetal calf serum (FCS ), # A-1115-L; I CN Biochemicals (Flow Labora tories): amphotericin B, #16-723-48; newborn calf serum (NCS), #29-121-54; try pan blue, # 19 5 5 32; Na/gene: Erlenmeyer flask, # 100-4116C; Pharmacia: Percoll, #17-0891-01; density marker beads, #17-0549-01; Sigma: gentam1cm sulfate, #G3632; penicillin/streptomycin, #P3539; trypsin inhibitor, #T9128; Tetko: HC- 160 nylon mesh, #3-160-53."

Dec 1, 1995

By Meng Li, Hao Nie, Weitian Ding, Mamdouh Omran, Ying Li, Yao Ge, Yaowei Yu, Abourehab Hammam, Lei Zan

This study examines the isothermal and non-isothermal reduction behaviors of iron ore compacts in a pure hydrogen atmosphere and compares the results obtained during the reduction process by CO. The different phases accompanying the reduction reactions were identified using X-ray diffraction (XRD) and its morphology was microscopically examined. In isothermal experiments, temperature plays a significant role in the reduction process. At any given temperature, the reduction rate during the initial stages is higher than that during the final stages. The reduction rate in H2 atmosphere was faster than in CO gas. The comparison of activation energy values suggested that reduction with H2 is more efficient thanwith CO. At the same temperature, the time required to achieve a certain degree of reduction was lower when using H2 gas than CO atmosphere. In non-isothermal tests, the heating rate has a significant effect on the reduction rate and reduction extent. At the same heating rate, the degree of reduction was higher in H2 atmosphere than in CO gas. Based on experimental data, the parameters of reaction kinetics were deduced by application of model-free and model-fitting methods. The reduction in H2 atmosphere was controlled by nucleation model (Avrami-Erofeev model), while the CO reduction reaction was controlled by gas diffusion.

Sep 29, 2020

By Mingyang Li, Yan Lv, Xiaolong Ma, Xudong Gao, Jie Zhao, Yingyi Zhang

This study aims to investigate the reduction thermodynamics of ilmenite and pre-oxidized ilmenite, and reveals the isothermal reduction kinetics of pre-oxidized ilmenite concentrate by carbon monoxide. The results indicate that the ilmenite concentrate and pre-oxidized ilmenite concentrate can be selectively reduced by controlling the temperature. The pre-oxidation process leads to the breakage of complex mineral structure, and the formation of porous simple oxides, which is an effective method to increase the reduction and leaching rate.