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By V. A. Marple, K. L. Rubow, Y. Tao

"A personal diesel exhaust aerosol sampler has been designed and evaluated in both laboratory and field settings. The sampler uses size selective sampling for the determination of the respirable mass concentrations of mineral dust and diesel exhaust particulate matter found in underground coal mines. This design is based on the premise that diesel exhaust particulate matter is predominantly less than 0.8 um while mineral dust is greater than 0.8 gm. Operating at a flow rate of 2 L/min, the sampler consists of a 10 mm Dorr-Oliver respirable dust cyclone followed by an 0.8 gm cutpoint impactor and an afterfilter. Field evaluations consisted of sampling at 6 different locations in these underground coal mines. Measured aerosol concentration less than and greater than 0.8 um have been compared with a well characterized cascade impactor.Techniques must be developed for measuring the mass concentration of diesel exhaust particulate matter found in underground coal mines due to the health concerns associated with worker exposure to diesel exhaust particulate matter. Diesel exhaust particles are in the respirable size range and have been regarded as a potential occupational carcinogen by NIOSH (1988). Furthermore, a 1988 MSHA advisory committee directed MSHA to develop a sampling protocol for assessing worker exposure to diesel exhaust particles.The University of Minnesota and the Bureau of Mines, over the past 4 years have measured the size distribution of diesel exhaust particles and mineral dust, both in the laboratory and underground mines (Cantrell, 1987; Cantrell et al., 1987; Cantrell and Rubow. 1988; Marple et al, 1986; Rubow et al., 1988: and Watts et al., 1989). To date, measurements have been made in nine coal mines. Seven mines utilized diesel-powered equipment at the mine face while two used electric-powered equipment. The results of these field tests indicate that mineral dust and diesel exhaust particles can be differentiated on the basis of particle size. The measured particle size distributions have been found to be bimodal with a saddle point at 0.8 um. This has led to the hypothesis that one method for measuring worker exposure to diesel exhaust particles would be to collect size fractionated particle samples separated at 0.8 um with the diesel particles being less than 0.8 um and the mineral dust particles greater than 0.8 um.Aerosol size distribution measurements, using a micro-orifice uniform deposit impactor (MOUDI), have been conducted in the mine intake air portal, intake entry to the working section, haulageway, and section return entry. The MOUDI is a ten stage cascade impactor with particle cutpoints ranging from 0.1 to 15 um aerodynamic diameter (Marple and Rubow. 1984b; Marple et al., 1989). The sampling flow rate is 30 L/min."

Mar 1, 1992

By R. L. Grayson, W. E. Wallace, T. Simonyi, J. C. Harrison

"Application of an SEM-based methodology suggests a reason for past contradictory results on the relationship between quartz and coal workers' pneumoconiosis (CWP). Results indicate that coal seams with the same bulk analysis of quartz may have different amounts of surface-availablequartz. Our research shows that the amounts of pure and surface-available quartz in a respirable dust vary directly with the rank of a coal seam, except in the case where rock materials are mined. The estimated amount of surface-available quartz therefore provides a measure of the quartz-related fibrogenic potential of a respirable dust.INTRODUCTIONFrom our previous work (Grayson, 1991), we have shown that the amount of pure quartz in a respirable coal mine dust sample appears to vary directly with the rank of the seam (using fixed carbon as the basis for comparison), except when a substantial rock parting, roof or floor are mined. Our other work (Wallace et al., 1989) showed that high-silica particles in a clay mine may generally be coated with clay, rendering the quartz core non-available for surface reaction in the lung. The combination of these findings led us to hypothesize that the coating phenomenon may indeed be present in coal mines as well, and that a rank relationship may be present regarding this phenomenon. Thus higher rank coal seams may have more surface-available quartz as well as more pure quartz particles in their respirable dusts. The scientific protocol for testing this hypothesis and the results obtained to date are discussed in this paper. Thus far, there is no reason to doubt that the relationship holds."

Mar 1, 1992

By Jianmin Qin, Raja V. Ramani, Wilson Miola

"The number of underground work entities operating under reduced dust standards in U.S. coal mines has been increasing since the start of the decade of the 80s and is currently approaching 10,000. The concern for controlling the quartz levels in airborne respirable dust in coal mine has manifested itself in several improvements in equipment selection and operation, face ventilation practices, and dust control procedures. At the same time, fundamental studies have been initiated on determining the source-sink relationships of the airborne quartz dust. The P-7 method is the prescribed method for the determination of quartz in the respirable airborne dust samples from coal mines. Questions have been raised on the reliability of the P-7 infrared spectrometry method for quartz analysis in powders. No specific method has been defined for quartz determination in bulk samples from the mine.In a recent study on the relationship between quartz in the airborne dust and that in the materials being mined, both infrared (IR) and x-ray diffraction (XRD) methods were used on a suite of samples from several coal mines. These samples encompassed airborne respirable dust samples, airborne total dust samples, the oversize fraction from the respirable samplers, ROM samples, channel samples, and chip samples from the face, roof, and floor materials. In this paper, the sample preparation and analysis procedures for the samples are discussed. The data, analysis results, and comparisons of the P-7 and XRD results are presented. The quartz percent determined for the same sample by the two methods are different. In 85% of the samples analyzed, the quartz percents determined by the XRD method are higher than those obtained by the P-7 method. However, there is a strong linear relationship between the results from the two methods. Further, the XRD method indicates an average of 20 percent more quartz than that from the P-7 method. The reasons for the differences in the results and the need for additional studies are explored.Since the enactment of the Federal Coal Mine Health and Safety Act of 1969 and the Federal Occupational Safety and Health Act of 1970, the prevalence of free silica as an occupational hazard in dusty work environments has been a matter of increasing concern. In underground coal mining, sometimes, roof, floor, clay veins and other non-coaly material must also be mined along with the coal. Mining these materials may increase the amount of quartz in the airborne dust (Pimentel and Adam, 1985; Taylor et al., 1984; Colinet et al., 1985). The problem of high quartz levels in the respirable airborne dust is widespread in continuous miner sections. In addition to the need for controlling the silica amount in mine atmosphere, a need for a sensitive, easily performed method for the determination of free silica in airborne dust samples was also recognized."

Mar 1, 1992

By D. H. Carlson, J. H. Johnson

"The U.S. Bureau of Mines and Michigan Technological University (MTU) are collaborating to develop an apparatus for measuring diesel tailpipe emissions in underground mines. A tailpipe emissions measurement apparatus (EMA) is described that dilutes diesel exhaust and measures the concentrations of diesel particulate matter (DPM), CO, CO2, NO, and NO2 at known dilution ratios.The EMA was evaluated by side-by-side comparison of its measurements with those by laboratory grade instruments. Concentrations of CO and C02 measured by the EMA were usually within ± 14 pct of concentrations measured by laboratory-grade instruments for CO and ±20 pct for C02• EMA measured DPM concentrations were, on average, 29 pct lower than those measured by laboratory-grade instruments, with the differences being fairly consistent. The CO and C02 differences are related to the method of calculating the EMA's dilution ratio, and the difference in DPM concentrations is attributed to thermophoretic and/or condensation losses related to the EMA's unheated sample probe. Preliminary analysis of data obtained with a heated sampling probe and modifications in the sampling procedures have indicated reduced variability in DPM and in the calculated dilution ratios."

Mar 1, 1992

By C. P. Fang, V. A. Marple, K. L. Rubow

"Studies of the flow fields and particle impaction characteristics are more complicated in multi-nozzle impactors than in single-nozzle impactors, since the jets of air must penetrate cross-flowing air to impinge on the impaction plate. Results of heat and mass transfer analysis are employed to describe the cross-flow pattern in multi-nozzle impactors. A cross-flow parameter has been derived to predict the deflection of the air jet by the cross-flow. This parameter is expressed as a function of the geometric parameters for the multi-nozzle impactor. DnN 4D,, where N is the number of nozzles, Dn the nozzle diameter and D, the nozzle cluster diameter. Empirical particle collection data show that multi-nozzle impactors operate satisfactorily, if the cross-flow parameter is less than a critical value of 1.2. Impactors operating with a cross-flow parameter larger than this critical value are shown to possess poor particle collection characteristics.Inertial impactors of many designs have been used for sampling aerosol particles. Impactors can be single stage, consisting of a nozzle and an impaction plate, or a cascade impactor with several stages in series. The simplest design for a stage is a single nozzle with a flat impaction plate. However, many designs use stages with multiple nozzles of identical size, which allows for compact impactors to be designed at rather substantial flow rates and moderate pressure drops.Nearly all of the theoretical work, and much of the experimental work, has been performed on single-nozzle impactors. The results of single-nozzle impactor analysis are often applied to multiple-nozzle impactors, which are assumed to be a group of individual nozzles, each operating as though they were single nozzles. However, an important fact is often ignored: the interaction of flow from adjacent nozzles can change not only the fluid mechanics in the impaction region, but also the locations of the jet stagnation points, and the impaction characteristics of each nozzle.The effect of multiple nozzles on the particle collection characteristics of impactors has been studied by a few investigators. May (1964), Fletch et al. (1967), Friedrichs (1968), and Vaughan (1986) reported that the quantity of particles in deposits of nozzles in the first two stages of the Andersen multi-nozzle cascade impactor were not equal. The inner deposits appeared to contain fewer particles than the outer ones. However, Vaughan (1986) in addition showed that many of the deposits in the outer three rows were missing on the first stage, even though the outer deposits that were present were larger than the inner deposits.May (1964) conducted experiments and explained the phenomenon of smaller deposits near the center than near the edge. He noted that in Andersen cascade impactors the air issuing from the nozzles moves radially outward along the narrow channel between the nozzle plate and the impaction plate. This flow at 28.31min -1 created a pressure difference of about 1 mm water between the inner and outer sections of the channel. The effect was most pronounced in the first two stages, where the pressure drop across the nozzles was also about 1 mm water. Here, the flow velocities in the nozzles, and hence the deposits, were smallest at the center and increased toward the periphery of the impaction plate. since the pressure drop across the nozzles was the smallest at the center and a maximum at the periphery. This effect was negligible in the lower stages because of a larger pressure drop across the nozzles. According to May (1964), this effect could be eliminated by increasing the nozzle-to-impaction-plate distance to three nozzle diameters and improving the nozzle arrangement to allow air flow to escape to the periphery more easily."

Mar 1, 1992

By E. V. Cilento, R. C. Laotz, K. A. DiGregorio

"PULMONARY ALVEOLAR macrophages (PAM) protect the lungs by providing a first line of defense against inhaled foreign debris and bacteria. In this role PAM phagocytize foreign matter, secrete enzymes that augment microbial killing and digestion, and produce reactive oxygen me-tabolites (2, 3, 18, 19, 22, 26). However, in addition to this antibacterial function, PAM participate in a diversity of functions, such as tissue repair, lipoprotein metabolism, and immunoregulatory activities (11, 22).Studies have suggested the existence of a heterogeneous population of PAM with respect to immunologic function, enzyme release, phagocytosis, morphological characteristics, expression of membrane receptors, ad-herence, and superoxide (O2•) release (4-7, 10, 16, 21, 23, 28). This heterogeneity has been observed by analyz¬ing subfractions of PAM separated by cell density frac-tionation. For example, using guinea pig PAM, Dauber et al. (7) showed heterogeneity in cell density and size, morphological characteristics, and cytochemical properties, whereas Holian and co-workers (16) reported variations in migration, chemotaxis, O2• release, and pinocytosis among subfractions collected. Similarly, O'Neill et al. (21) observed functional heterogeneity in rat PAM with regard to phagocytosis, O2 • release, and lysosomal enzyme activity.Several investigations have reported a correlation between PAM activity and cell volume. Most studies have shown that O2 release from PAM increased with decreasing cell size (15, 21, 25), although Bielefeldt Ohmann and Babiuk (4) reported a decreasing trend. However, Gallin (13) has suggested that significant variability may even exist within density-defined or cell-volume subfractions that could lead to misinterpretation of results concerning PAM heterogeneity.A better understanding of the heterogeneity in O2 production may be obtained by using single cell meas-urements to evaluate whether functionally distinct sub-populations actually do exist. As such, the present study was designed to examine heterogeneity in O2 • release from single PAM using a recently developed electrooptical technique (8). The initial oxygen metabolite, O2•, produced by NADPH oxidase at the surface of the plasma membrane, was measured by quantifying the reduction of nitro blue tetrazolium (NBT I to a diformazan precipitate. Measurements were made from video recordings of individual cells stimulated by their adherence to the bottom of a culture dish. The cell volume and degree of spreading also were determined directly from the same video recordings, and the data were correlated with the initial rate and maximum amount of diformazan produced by the same cell."

Mar 1, 1992

By R. Burrell, N .. L. Lapp

"Except for asthma and pulmonary infections, the types of lung disease that have been of immunologic interest the longest are the various mineral pneumoconioses. Because of their chronicity, type of pathology produced, and well-known immunotoxic properties associated with the dusts inciting them, the pneumoconioses have long drawn attention to the possibility that the immune system has not only been modulated but that its effectors have possibly contributed to their pathogenesis. All of the mineral dusts reviewed here have definite immunomodulating properties and act on various cellular and molecular components of the immune system. Further, each has an immunotoxic potential to induce either hypersensitivity (as in the case of beryllium) or other types of immune injury. This article reviews the immunologic aspects of the four major occupational minerals that have an immunologic impact on the lung: asbestos (particularly chrysotile), silica or quartz, beryllium, and coal mine dust.These agents have many things in common, yet each is distinctive. Although the possibility still exists that these mineral particles can adsorb intrinsic tissue antigens or extrinsic organic antigens to their surfaces and initiate immune responses to the adsorbed antigens, only one, beryllium, can act as an antigen itself. Silica is a profound macrophage toxin and thereby exerts many effects on those factors controlled by macrophages. Asbestos has undergone the most immunologic studies resulting in a much dearer picture of how it may contribute to immunopathology. Coal mine dust is the agent about which least is known immunologically, partly because there is no such thing as purified coal mine dust or a standard by which coal mine dust can be defined. Indeed, there are studies that use only the dust from the ground coal itself, whereas other studies use the total dust collected from air samples of a working mine in the belief that other types of dusts (e.g., silica from roof bolt drilling, from sand ground on the rails by cars, and from surface drilling) contribute to the disease (Figs. 1 to 5). This properly is coal mine dust and is poorly characterized. Yet, workers exposed to these dusts share similar reactive characteristics and they may be considered collectively as well as individually."

Mar 1, 1992

By Danqing Xu, A. W. Khair

"This paper presents a laboratory simulation of regrinding and resulting dust generation. An Automated Rotary Coal Cutting Simulator (ARCCS) was modified to conduct regrinding experiment. In order to estimate the effects of coal properties and operating parameters on regrinding and dust generation, an orthogonal fractional factorial experimental design was implemented in regrinding experiment. Marple personal cascade impactors, Series 290, were used to measure dust concentration during experiment. Three types of coal seams, namely, Waynesburg, Pittsburgh, and Lower Kittanning, were used to represent different coal properties. Experimental results and complete parametric analysis were presented.In a rotary cutting system, all the broken coal and part of the remaining coal from previous cutting cycles must be transported through part or entire circumference of the cutting drum. This transportation of already recovered coal through the cutting zone of rotary head makes it a rotary grinder. Some of the coal particles will be subjected to secondary fragmentation or regrinding in the process. The potential effect on dust generation by regrinding is significant because the cutting head utilizes kinetic energy to comminute recov¬ered coal material. The moving bits and bit blocks on the cutting head impact coal particles and transfer some of their kinetic energy to the particles. After obtaining kinetic energy, coal particles are free to impact one another and they also impact the coal seam or cutting head. During impact, high impact force and high internal stress concentration are created in the particles. Kinetic energy is transformed into strain energy. When stored strain energy exceeds allowable strain energy, the particles will break. A great amount of respirable dust will be generated due to impact failure. In reality, regrinding occurs at the same time as primary cutting. The particles to be ground are the result of primary cutting. Both primary cutting and regrinding produce dust. Therefore, it is impossible to separate regrinding from primary cutting. In order to study respirable dust generation by regrinding and analyze the influencing parameters, a laboratory approach was adopted. In this approach, it is possible to study the regrinding separating from primary cutting."

Mar 1, 1992

By N. S. Dalal, Xianglin Shi

"The mechanism of vanadium(V) V ) toxicity is still not fully understood in spite of extensive current effort (1-3). The presence of a vanadium(V) V ) -dependent NAD ( P )H oxidase in cell membranes suggests that at least one of the pathways involves oxidation of NAD(P)H (1-3). Considerable effort has therefore been devoted to finding the mechanism of NAD ( P ) H-oxidation by va¬nadium( V ) (1-19). Recently Liochev and Fridovich (4, 16) provided a critical review of vanadium( V) -stimulated NAD (P )H oxidation by plasma membranes. They out¬lined a mechanism in which vanadium(V) V )-stimulated oxidation of NAD ( P )H by biomembranes involves an O2 - initiated free radical chain reaction (4 ). While the above may be the major pathway for the vanadium-me¬diated NAD (P )H oxidation in membrane-related sys¬tems, some recent results suggest that in the presence of NADPH the flavoenzyme glutathione reductase can function as a vanadium(V) V ) reductase (18). Because of the significance of this result in relationship to the mech¬anism of vanadium( V) metabolism and the novelty of the metal-reductase behavior of a flavoenzyme, we have car¬ried out additional studies on one-electron reduction of vanadium( V) by two other, related flavoenzymes: lipoyl dehydrogenase and ferredoxin-NADPH oxidoreductase. These two enzymes were selected because they are known to catalyze a one-electron reduction of Cr ( VI ) (19-21 ), which is isoelectronic with vanadium( V ).The present study also reports on the generation of hydroxyl ( • OH) radicals in the vanadium( V) -flavoen-zyme redox system without exogenous H2O2. The • OH study was undertaken because • OH radicals are known to be generated in reactions of vanadium( IV) with H2O2 (17,18,22-27). For example, Piette and co-workers (17, 22) have studied effects of vanadium(IV) IV ) on lipid per-oxidation and NADPH oxidation and suggested that va-nadium( IV) -induced • OH radical formation may play an important role in vanadium( V) toxicity. Carmichael (25, 26) investigated the reaction between a RNA /VO 2' complex and H2O2 with a view to probe the possible re-lationship between vanadium( IV) -mediated • OH radical generation and DNA damage. He has suggested that in this reaction the • OH radical adds to the C (5) carbon of uracil, causing nucleotide damage, similar to what occurs in the nucleotide /Fe2+ / H2O2 reaction (28) and in y-irradiated aqueous solutions of nucleic acids (29 ). To our knowledge, however, there is as yet no report on ·OH radical generation in any reaction involving the reduction of vanadium ( V) by a well-characterized enzyme, without exogenous H20 2 • The present study provides evidence that ·OH radicals can form in a flavoenzyme-mediated reduction of vanadium( V) to vanadium( IV) and that this process involves also the reduction of molecular oxygen ( 0 2 ) to H20 2 • The H20 2 thus generated reacts with vanadium( IV) to produce ·OH radical, in analogy with the Fenton reaction ( Fe2 + + H20 2 - Fe3 + + OH- + ·OH)."

Mar 1, 1992

By V. A. Marple, K. L. Rubow, S. M. Behm

"An eight-stage cascade impactor, with cut-sizes ranging from 0.056 to 18 um at a flow rate of 30 L/min, has been developed, calibrated, and tested in field programs. This impactor, called a microorifice uniform deposit impactor, has several features not normally found in cascade impactors. Ultrafine particles as small as 0.056 um are collected while keeping the pressure drop moderate by utilizing multiple nozzles (up to 2000) as small as 52 um in diameter. At each stage the collected particles are deposited uniformly over the entire impaction plate by rotating the impaction plate relative to the nozzles and by the correct radial placement of the nozzles. The particle cutoff characteristics (collection efficiency curves) of each stage and inter-stage losses were determined by means of monodisperse aerosols. The cutoff characteristics were found to be sharp and similar for all stages.Inertial impactors are widely used for the size-selective collection of aerosol particles. One reason for their wide usage is that the principle of operation is simple: a jet of particle-laden air is directed at a flat impaction plate. Large particles are collected on the plate while smaller particles follow the airflow out of the impaction region and are not collected. The division between the size of particles collected and those that are not is quite sharp. Secondly, the particles in an impactor are classified by their aerodynamic diameter (an important size parameter in many fields of study, including those related to respirable particle health effects). Thirdly, it is easy to collect particles in discrete size ranges by passing the aerosol through a number of stages (one stage consisting of a nozzle and impaction plae) in series, with each subsequent stage collecting particles smaller than the one before it. This is known as a cascade impactor. Finally, impactors can classify particles over a wide range of particle sizes: as low as 0.005 um (Fernandez de la Mora et al., 1990) and as large as approximately 50 um (Vanderpool et al., 1987).This paper describes a general purpose cascade impactor that has been developed in our laboratory and used in a variety of studies including visibility studies (McMurry et al., 1986; McMurry and Zhang, 1989), underground mine studies (Marple et al., 1986; Rubow and Marple, 1988; Rubow et al., 1988), and general atmospheric pollution studies (Macias et al., 1981; Dzubay et al., 1984). The impactor, called a microorifice uniform deposit impactor (MOUDI model 100, MSP Corp., Minneapolis, Minn.), is the integration of two earlier impactor de¬velopments: the microorifice impactor (M01) (Kuhlmey et al., 1981) and the uniform deposit impactor (UDI) (Marple et al., 1981).The MOUDI has several features normally not found in other cascade impactors. These include collection of particles as small as 0.056 um in aerodynamic diameter with a moderate pressure drop, uniform particle collection on the impaction plates, inter-changeable impaction plates to allow for the plates to be easily and quickly changed in the field, and covers for these substrates to provide a means for safe storage and/or transport of the collected particles to the laboratory for analysis."

Mar 1, 1992

By R. V. Ramani, J. Qin, R. A. Jankowski

"Longwall mining is generally recognized as safer, more productive and economic when compared to room and pillar underground coal mining. There has been, however, increasing concern for the control of airborne coal dust concentrations in long wall faces. The airborne dust cloud at a longwall face is a complex aerosol system containing particles of varied size, density, shape and state of aggregation. For the development of more effective dust control practices in longwall faces, it is necessary to understand more clearly the characteristics of the longwall dust cloud.In this paper, results of a research study on dust concentration distributions along longwall faces are presented. The objective of this study is to characterize the longwall dust cloud behaviour and provide necessary fundamental data for a better understanding of the longwall dust problem. MSA personal samplers and gravimetric total dust samplers were employed to collect respirable and total airborne dust samples. The size distribution of these samples was determined by MicroTrac SPA. The instantaneous respirable airborne dust concentrations were recorded using RAM-I. The dust concentrations are related to face operations, particularly the shearer location and activities. The size distribution of airborne dust, instantaneous respirable airborne dust concentration, and air quantity distributions along longw all faces reveal a complex dust flow phenomenon. Additional fundamental studies are needed for characterizing the temporal and spatial behaviour of the dust cloud at longwall faces.IntroductionModern mechanized longwall mining was introduced in the United States in the 1960's and has become more common in the last twenty years. United States longwalls are multiple-entry retreating faces. According to a recent survey, 91 of the 102 longwall faces employ double-drum shearers (1). Several recently installed longwall faces have consistently produced over 4 000 to 5 000 tons per shift over extended periods of operation. The trend in under-ground coal mining in the U.S. certainly is toward the introduction of longwalls. This trend is true not only for new mines but also for renovation of existing mines. While longwall mining has many advantages over other mining methods, including improved safety, such as higher pro-duction. greater productivity and enhanced reserve recov-ery, it is associated with severe problems of dust control. The increase in longwall production has been accompanied by an increase in respirable dust problems which limit realizing the full potential of the longwall mining system. Despite the efforts to develop improved equipment and operational procedures to control the dust levels at longwall faces, it is still difficult to bring several double-drum shearer operations into compliance with the Federal stand¬ards of 2,0 mg/m 3 respirable dust without loss of produc¬tion. In fiscal year 1987, fifty-eight of the longwall sections were found to be in non-compliance once, and an additional 31 were cited two or more times'''. If the full potential of the longwall mining method is to be realized, new measures must be implemented to control the dust concentration to mandated levels. There is a need for detailed and extensive sampling of longwall faces to understand the dust source-sink relationships and the transient ambient concentrations. These must be related to the mining activities on the face. This paper is to characterize the longwall dust cloud behavior through a series of experiments in operating faces and provide necessary fundamental data for further studies to develop improved dust control practices."

Mar 1, 1992

By B. K. Cantrell, K. L. Rubow

"Two source apportionment techniques have been applied by the U.S. Bureau of Mines to aerosol measurements in diesel-equipped underground noncoal mines. The first technique is based on size-selective sampling and the premise that the diesel exhaust fraction of the aerosol is predominately submicrometer in size while the mineral dust fraction of the aerosol is mostly supermicrometer in size. The second technique, chemical mass balance (CMB) modeling, was used to referee the analysis of diesel and mineral dust aerosol concentrations with the size selective method.The size-distribution data were modeled using a lognormal regression to parameterize the submicrometer and supermicrometer fractions of the sampled aerosol and to estimate the mineral and diesel contributions to each. Using this analysis, 97 ± 2 pct of the submicrometer aerosol mass was attributable to diesel exhaust aerosol.The CMB analysis, applied to both fractions of the respirable mi11e aerosol, yielded the amount contributed to each by the diesel and dust aerosol sources. On the basis of this analysis, 94± 10 pct of the submicrometer fraction was diesel exhaust aerosol. As much as 20 pct of the diesel aerosol, however, was found in the supermicrometer fraction, requiring a correction of the submicrometer fraction results to account for the missing diesel mass. More accurate measurements will require a carbon-specific analysis of the aerosol.INTRODUCTION Measurement of the contribution of diesel exhaust to respirable aerosol in mine environments has become increasingly important because of current concerns over the occupational health effects resulting from exposure to diesel emissions. In response to this, the U.S. Bureau of Mines is developing and evaluating new sampling methods for measuring diesel aerosol in underground mines. Two such techniques are being studied by the Bureau, size selective sampling and CMB modeling. These techniques use measurable physical or chemical characteristics of a mine aerosol sample to infer the amount of diesel particulate material contained in the sample."

Mar 1, 1992

By Raja V. Ramani, Wilson Miola

"The potential health effects of quartz-bearing airborne dust in mine and mill atmospheres point to the need for the quantification of quartz content in the source material for determining the source-sink relationships and for choosing applicable control measures. While a standard method exists from a regulatory point of view for the sampling of coal mine atmospheres for determining (i) respirable dust concentrations and (ii) the amount of quartz in the samples, there is no generally accepted standard method for quartz analysis in bulk samples of coal and host rocks. Generally, the bulk sample is reduced to a powder for producing quartz analysis samples. Extensive controls arc necessary to ensure representativeness of the analysis samples when these are very minute fractions of the bulk samples. Since the amount of analysis sample is not limited, alternative methods for quartz analysis may also be tried. It is, however, necessary to adopt a procedure which will satisfy several statistical requirements to guarantee representativeness of the quartz analysis samples.In this paper, the important factors in the preparation of quartz analysis samples from the host rocks and bulk samples are discussed. The method developed to prepare the analysis samples is outlined. The results of application of the procedures are presented. The procedure developed for quartz analysis sample preparation is based on sound statistical foundations. The procedure has been validated for reproducibility and homogeneity.The current methods used for quartz analysis in powders are the P-7 infrared spectrometry and quantitative X-Ray Diffraction (XRD). Both have similar restrictions concerning the sample characteristics and operational problems, which arc intrinsic to radiation beams, device geometry, mechanics and controls of the radiation behavior. Although improvements can be made in the spectrometers and diffractometers, some of the most important steps are the procedures for analysis sample preparation from bulk samples and for calibration of the instruments. The sample preparation process is often referred to as one of grinding and sizing the bulk sample to generate the powder for the scanning devices. The particle sizes are referred to and their influence emphasized but the representativeness of the sample is assumed. Assuring sample representativeness during the sample preparation procedures is critical.For the sampling of airborne respirable dust in coal mines, the collection devices, the collection procedures and the analysis procedures are defined. This is not true for bulk samples where weights must be reduced thousands of times to generate the analysis samples for the scanning devices. The specifications to be developed may include bulk sample amount, sample reduction procedures, sample particle size distribution and sample amounts. However, the common problem of insufficient sample amount which is often encountered with mine airborne dust samples need not exist for bulk samples. Further, the same bulk sample can yield analysis samples for several experiments. The latter, of course, requires sample homogeneity.Three concepts are important in the selection of any procedure for sample preparation and analysis. These arc accuracy, reproducibility and representativeness. The adopted procedure has to guarantee a homogeneous sample representative of the population, a defined level of accuracy and reproducibility. Important statistical concepts are embedded in these requirements, particularly with respect to estimation and dispersion errors. Accuracy and reproducibility are totally different though not independent concepts. Estimation is related to accuracy and the true value. Dispersion is related to reproducibility and is measured by the classical statistical standard deviation of the distribution of a series of measurements or analysis performed over the same lot or analysis sample. The dispersion variance (or dispersion error) does not tell a"

Mar 1, 1992

By J. M. Mutmansky

"This study analyzed laboratory comminution techniques for respirable mine dust generation and the properties of the resulting dusts. It was the objective of the study to determine the suitability of the resulting dusts in reflecting the properties of natural airborne dust. This paper deals specifically with the amount of contamination introduced into the generated dust as a result of abrasion when using nine different grinding methods. The analysis was made by comparing the content of 25 chemical elements in the lab-generated dusts with the same elements in the airborne coal mine dusts. It was found that all the lab-generated dusts possessed contamination but that several methods produced smaller percentages of contamination and that the residence time in the grinding device was an important factor in the contamination levels. INTRODUCTIONDust in the atmosphere is a health hazard and a major source of occupational diseases in the coal mining industry (Anon., 1980; Merchant, Boehlecke, and Picket-Hamer, 1986). It has been shown that coal workers' pneumoconiosis (CWP) is directly related to the inhalation and accumulation of respirable coal dust in coal miners' lungs. In order to effectively protect coal workers from coal dusts, we must have a clear understanding of dust properties including dust generation and dust-lung reaction mechanisms. This requires extensive biomedical research. Dust related research often requires large samples of respirable coal dusts to be collected, either for repeated experiments or stored for future reference. Unfortunately, current sampling instruments are not able to meet this demand. Ali a result, laboratory-generated coal dust ls frequently used as a substitute for coal mine dust in biomedical and other research applications. Now, the questions are how close the characteristics of laboratory-generated dusts are to those of mining-generated dusts and which equipment is moat appropriate for research dust production in the laboratory . These are some of the interests of this research project. The primary objective of this project was to find a laboratory technique which can generate coal dusts possessing properties similar to coal mine dust with respect to particle size distribution, chemical composition, and particle shape and morphology. However, this paper will focus only on the chemical compositions of the prepared dust samples and try to identify the sources of chemical contamination from various size reduction processes."

Mar 1, 1992

By H. Polat, Q. Hu, S. Chander

"The collection efficiency of dust particles was determined in a laboratory dust chamber equipped with a water spray. Measurements were made in the presence of selected nonionic, cationic and anionic surfactants. For the nonionic and the anionic reagents, efficiency was maximum in a narrow range of concentration. The surfactant concentration corresponding to high collection efficiency was usually much below the commonly used concentration of l "", however. In comparison, much higher collection efficiency was observed for a cationic surfactant at extremely low concentrations. For delineating the mechanism of capture of dust particles, surface tension and contact angle results were used to obtain a correlation between the work of adhesion and the efficiency of collection. Such a correlation was reasonable for the nonionic surfactants but not for charged surfactants. In the latter case, electrostatic interactions between charged droplets and particles were more important, especially for the cationic reagent. INTRODUCTIONSeveral methods are used to control dust in mines. These include: ventilation, foam, scrubbers and sprays. This article is concerned with improving the effectiveness of water sprays, which are widely used to remove dust particles during continuous mining, by adding small quantities of surfactants. Water sprays produce small droplets which act as collectors of dust particles suspended in air. Sprays directed at the actual source of dust have the additional property of wetting the coal before the ·particles become airborne (Hamilton, 1974). Wetting agents, also called surfactants, arc known to play an important role in many applications where dust is a problem. However, conflicting reports exist in the literature with regard to the effectiveness of surfactants in water sprays (for a review, see Chander et al., 1988). In spite of the conflicting reports, the use of surfactants for dust control has increased over the years. In a recent study by the Bureau of Mines, a 27'6 reduction in respirable dust levels was observed in a mine test when surfactants were added to sprays (Foley, 1991)."

Mar 1, 1992

By E. D. Regad, W. E. Wallace, P. S. Mike, C. A. Hill, V. Vallyathan, M. J. Keane

"Respirable-sized quartz, a well-established fibrogenic mineral dust, is compared with kaolin in erythrocyte hemolysis assays aher treatment with saline dispersion of dipalmitoyl phosphatidylcholine, a primary phospholipid component of pulmonary surfactant. Both dusts are rendered inactive after treatment, but the membranolytic activity is partly to fully restored aher treatment with phospholipase Ai. an enzyme normally associated with cellular plasma membranes and lysosomes. Phospholipid-coated dusts were incubated for periods of 2-72 h at a series of applied enzyme concentrations, and the adsorbed lipid species and hemolytic activity were quantitated at each time for both dusts. Surfactant was lost more readily from quartz than from kaolin, with consequent more rapid restoration of mineral surface hemolytic activity for quartz. Interactions of surfactant and mineral surface functional groups responsible for the mineral-specific rate differences, and implications for determining the mineral surface bioavailability of silica and silicate dusts, are discussed. INTRODUCTIONMineral particles depositing in the lung acinus will contact an aqueous coating containing surfactant molecules spread on the air-liquid interface and dispersed within this hypophase; the primary components of that surfactant are diacyl lecithin phospholipids (King and Clements, 1972; King, 1982). Adsorption of this surfactant can suppress the cytotoxicity of mineral dusts (Marks, 1957). Suppression of hemolytic activity, a measure of membranolytic potential and an indicator of bioavailability of the mineral surface, of respirable-sized quartz and kaolin clay dusts with the adsorption of dipalmitoyl glycerophosphorylcholine or dipalmitoyl lecithin (DPL) has been measured (Wallace et al., 1985). Such contact and adsorption of pulmonary surfactant in vivo may provide a first defense system of the lung against respired dusts."

Mar 1, 1992

By M. S. Seehra, R. S. Pandurangi

"INTRODUCTION Silica and kaolin (a layer aluminosilicate) are among the major constituents of most coal mine dusts.' Whereas kaolin is cytotoxic in vitro but nonfibrogenic in vivo in humans and experimental animals,2-4 the cytotoxic and fibrogenic potential of silica is rather well established.5 Wallace and co-workers 6-8 have studied the cytotoxic potentials of both native and surface-modified silica and kaolin and inferred that retoxification is slow in the case of occluded silica vis-à-vis native silica. Recent epide¬miological studies indicate that silica concentration alone in a mine dust, as determined by transmission IR spec¬troscopy (TIRS), does not correlate well with the pro¬gression of coal workers' pneumoconiosis. 9 This obser¬vation has led to the suggestions that silica particles occluded by clays such as kaolin may not be fibrogenic. This proposal requires accurate estimations of kaolin, silica, and occluded silica. The current method of silica analysis in a mine dust sample employs TIRS of the 797- cm -1 band of silica, although its accuracy, as well as that of x-ray diffraction studies, is no better than 25 % , even in a laboratory-generated dust.° In this paper we have employed the techniques of diffuse reflectance spectros¬copy (DRS) and photoacoustic spectroscopy (PAS), in conjunction with an FT-IR spectrometer, for the quan¬titative determination of silica in laboratory-generated silica-kaolin mixtures.One of the main advantages of DRS and PAS is that, in these techniques, problems arising from sample prep¬aration of the KBr-based disks used in TIRS are avoided. There are, however, several other considerations (e.g., particle size effects and saturation phenomenon) which complicate the use of DRS and PAS for quantitative work. 10-25 In this paper, we have carried out a systematic comparison of the quantitative abilities of the two tech¬niques, as applied to the silica-kaolin mixtures, with par¬ticular attention to the saturation behavior of several bands with different absorption coefficients. In particular, we show that a weaker band for silica at 1875 cm-1 is suitable for quantitative purposes, and that quantitative accuracy is higher in DRS than in PAS. Details of this work are given below."

Mar 1, 1992

By L. M. Demers, D. C. Kuhn

It has been suggested that radicals on the surface of dust particles are key chemical factors in the pathophysiology that results from the occupational inhalation of coal and silica dust. In addition, oxygenated derivatives of arachidonic acid (eicosanoidsJ have been implicated as important biochemical mediator5 of mineral dust-induced lung disease through their role in bronchial and vascular smooth muscle reactivity, inflammation, and fibrosis. Therefore, we assessed eicosanoid production by the rat alveolar macrophage (AM) exposed in vitro to mineral dusts with varying surface chemical characteristics in order to determine if radicals associated with the mineral dust could influence the production of proinflammatory mediators in the lung environment. Primary cultures of rat AM were exposed to freshly fractured or "stale" bituminous coal dust, as well as untreated silica or silica calcined to 500 and 1100°C. Prostaglandin E1 (PGE;V, thromboxane Ai (T}(Ay, and leukotriene 84 (LTB; levels in incubation medium were determined by specific radioimmunoassay. When AM were exposed to freshly fractured coal dust, PGE2 production was markedly increased. In contrast, exposure of AM to "stale" dust significantly reduced PGE1 production. Exposure of AM to freshly fractured coal dust resulted in a significant increase in production by AM, while exposure to stale coal dust did not influence AM TXA1 production. Neither "fresh" nor "stale" coal dust had any effect on LTB4 production. In vitro exposure of AM to untreated silica resulted in a significant increase in TXA1 PGE.,, TXA; and LTB4 production compared with control. However, exposure of AM to silica calcined to 11oo•c resulted in eicosanoid levels that were not significantly different from control. These effects were still apparent 8 wk after calcination of the silica particles. Silica was a more potent activator of AM eicosanoid production than was coal, and amorphous fumed silica was a more potent activator of AM eicosanoid production than was crystalline silica. These findings suggest that radicals associated with respirable coal and silica particles may play a key role in the ability of mineral dust to activate AM eicosanoid production and therefore may be important in the pathophysiological consequences of occupational mineral dust inhalation.

Mar 1, 1992

By D. L. Nussear, W. E. Wallace, N. Clark, M. Gautaum, G. Hong-Guang

"Results of a research effort directed towards identifying and measuring the genotoxic properties of respirable particulate matter involved in mining exposures, especially those which may synergistically affect genotoxic hazard, are presented. Particulate matter emissions from a direct injection diesel engine have been sampled and assayed to determine the genotoxic potential as a function of engine operating conditions. Diesel exhaust from a caterpillar 3304 diesel engine, representative of the ones found in underground mines, rated 100 hp at 2200 rpm is diluted in a multi-tube mini-dilution tunnel and the particulate matter is collected on 70 mm fluorocarbon coated glass fiber filters as well as on S"" x 10"" hi-volume filters. A six mode steady state duty cycle was used to relate engine operating conditions to the genotoxic potential. Results to-date on diesel exhaust have indicated that use of a primary component of pulmonary surfactant as a medium for collecting and applying respirable particulate material to cells for mutagenicity testing offers a true representation of in-vivo delivery of such respired aerosols to pulmonary cells and the genetic material within. Protocols have been developed to sample and assay the diesel soot and diesel soot/mineral dust complexes which would not compromise the surf ace and physical properties of the particulate material. Evidence has been gathered to show that diesel soot expresses mutagenic potential as dispersions in DPL in the Ames tests as well as sister chromatid exchange activity in the animal lung fibroblast ·derived V79 cell line. The results are comparable to the standard solvent extraction procedure. INTRODUCTIONDiesel engines are primarily associated with transportation; however, they are also very common in the work place. Of particular concern is the use of diesel engines in mining operations. Currently there are 1,950 diesel machines working in underground coal mines throughout the country. These units are used in 151 mines encompassing nine different states (MSHA, 1991). It is believed the use of diesel powered equipment has contributed to higher productivity in these mines. However, the exhaust from diesel engines operated in underground mines has been a subject of concern during the past decade because of its suspected relationship with health aspects. The particulate matter generated by the diesel engine is of particular concern."

Mar 1, 1992

By D. A. Lucero, J. E. Brockmann, L. A. Mondy, K. L. Rubow, D. J. Rader

"Department of Mechanical Engineering, University of Minnesota, Minneapolis, MNExperimental and correlated stage responses (the fraction of particles entering an impactor that are collected on a stage) are presented for the Andersen Mark III and Marple personal cascade impactors. The impactors were operated upright and fully assembled so that interstage interference and wall losses could be properly studied. The observed stage responses showed maxima that fell significantly short of unity, meaning that a monodisperse aerosol is never collected exclusuvely on one stage, but is distributed among several stages and internal losses. Correlations for the stage responses are presented so that the experimental results can be used to determine size distributions with available data-inversion algorithms. Simulations with log-normal distributions show significant differences between dpa50 histograms and the more accurate distributions that result by taking the response functions into account.Cascade impactors are widely used for the measurement of aerosol size distributions; their ruggedness and simplicity of operation make them an attractive choice for a variety of environments. In one common application, the aerosol of interest is drawn through the impactor, and the amount deposited on each stage is determined gravimetrically. The primary objective is to extract as much information as possible about the initial size distribution from the resulting set of weights. Unfortunately, the reconstruction of contin-uous size distributions from finite data sets is an ill-posed problem. Not surprisingly, a variety of such reconstruction techniques are presently in use; the success of each method depends on the particular nature of the aerosol sample and the instrument response.Inversion of impactor data by any of these techniques requires that the response functions for each stage be known. The stage response function gives the fraction of particles entering the impactor that deposits on that stage. Values of the response function range from zero to one, and depend on impactor operating conditions (e.g., geometry, flow rate, and gas properties) and particle properties (e.g., diameter, density, and shape factor). As interstage interferences and particle losses will affect each stage's response, experimental determination of stage responses should be made for fully assembled impactors. Unfortunately, impactor stage response functions are rarely reported; rather, single-stage efficiency curves are given that provide an incomplete description of impactor performance.This paper presents experimental stage responses for two fully assembled cascade impactors: the Andersen Mark III and Marple personal impactors (model 298, Andersen Samplers, Inc., Atlanta, Ga.). These calibrations were performed with monodisperse liquid droplets for both impactors and also with monodisperse solid spheres for the Marple personal impactor. The calibration results for the Mark 111 impactor presented here have been previously reported in the response-function format (Rader et al., 1989). The calibration results for the Marple personal impactor, however, were originally presented in the single-stage efficiency for¬mat (Rubow et al., 1987) and have been reanalyzed in this work. Losses within both impactors are considered, and their treat¬ment within data inversion techniques is dis¬cussed. In addition, correlations for each impactor's response functions are presented so that these calibration results can be easily included in data-inversion algorithms. Size distribution inversions using these response correlations are compared with the standard cut-point method for two lognormal test dis¬tributions."

Mar 1, 1992