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By N. S. Dalal, X. Shi, V. Vallyathan

"Electron spin resonance (ESRJ spectrum of the hydroxyl (· OH) radical spin adduct with .the spin trap 5,5-dimethyl-1-pyrrolintt-N-oxide has been obtained in suspensions of freshly ground quartz particles. The concentration of the spin adduct (and hence of the • OH radicals) increases with the amount of grinding. The dust's potential for the generation of the· OH radicals is maximum when fresh (i.e., immediately after grinding) and decreases to 50% in about a day on storage in air. Studies involving metal chelates .indicate that the • OH radical formation involves mainly the silica surface and H20 rather than the Fenton reaction. The results suggest that hydroxyl radical reaction(s) could be important in the lipid peroxidation and fibrogenicity by quartz dust, particularly .in acute silicosis. INTRODUCTIONIn this paper we report on our electron spin resonance (ESR) detection of the formation of hydroxyl (·OH) radicals in aqueous suspensions of freshly crushed quartz particles. This work was undertaken with a view to find possible clues to the role of free radicals in the pathogenesis of quartz-induced fibrogenicity, commonly known as silicosis. We noted that while silicosis is one of the major health hazards in coal and mineral industries, the mechanism by which the quartz particles exert their toxic action on cells and the process(es) by which these actions progress to fibrogenesis are still poorly ·understood (Reiser and Last, 1979; Singh and .Rahman, 1987). It is generally thought, nevertheless, that the action of quartz particles on the biological membranes is the starting point of the silicotic process (Parazzi et - al., 1968). A possible clue to the mechanism of the membrane damage by quartz particles, in terms of the role of oxygenated free radicals, is suggested by the following observations. A suspension of quartz particles in contact with alveolar macrophages has been reported (Gabor et al., 1975; Koike et al., 1982) to initiate an enhancement of lipid peroxidation, defined broadly as the oxidative deterioration of polyunsaturated components of lipids. Hydroxyl (· OH) radicals are known to be capable of peroxidation by abstracting hydrogen atoms from cell-membrane lipids (Halliwell and Gutteridge, 1985) and of initiating lipid peroxidation in lysosomal membranes. (Fong et al., 1973). Arong other lines, it is known that exposure of cell membranes, fatty acids, and unsaturated food oils to ionizing radiation, which generates· OH radicals, causes rapid peroxidation (Halliwell and Gutteridge, 1985). Thus • OH radicals (and also other oxygenated species such as Oi, 10 2, and H20 2) might be expected to play a role in the toxicity of quartz. Earlier studies of the aqueous chemistry of quartz suspensions have reported detection of H2O 2 (Kalbanev et al., 1980), implicating the formation of ·OH radicals as transient species. However, we are not aware of any report of the detection of • OH radicals in quartz reactions, and thus the present study was undertaken. Since it-is known that, because of their high reactivity (hence short lifetime) in aqueous media, the • OH radicals cannot be detected via ESR directly (Finkelstein et al., 1980; Buettner, 1982) we have used .ESR combined with the spin-trap methodology (Finkelstein et al., 1980) for studying the· OH formation. The results show that· OH radicals are indeed formed in aqueous suspensions of freshly ground quartz; thus pointing to ·a possible new clue to the cytotoxicity of freshly formed quartz particles and thereby to silica fibrogenicity."

Jan 1, 1988

By David A. Monaghan, Kenneth R. Maser, Adi R. Gurdar, D. Randolph Berry

APPENDIX D DEVELOPMENT AND EXPERIMENTAL VERIFICATION OF ACOUSTIC SEAL CHECKER THEORY 1. Introduction In Section 7 of the report the acoustic seal checker concept was described in a general manner and the parameters which affect the signal level at the microphone were discussed briefly. In this appendix the theoretical basis will be presented and the experimental program described which permitted the extension of the theory into practice. The basic goal of the acoustic seal checker is to relate the reduction in sound pressure level to the size of the residual hole in the fly ash barrier. The smallest hole size that may be measured is determined by the signal to noise ratio, where the noise in this case includes the ambient noise in the mine, the internal microphone noise and the sound transmitted from the acoustic source via the flanking paths through corridors adjacent to the one being sealed. The latter source of noise can be discriminated against, at the expense of increased complexity of the acoustic system, by employing a pulsed source and pulse gating of the microphone signal set to accept sound via the barrier path and to reject the delayed flanking path signals. In order that the acoustic system be insensitive to precise source and microphone location and to permit assessment of system performance on an acoustic power flow basis, a broad frequency band of pseudo-random noise is emitted by the loudspeaker source. The preferred frequency range must be determined, the advantage of a directive source must be assessed, and the necessity

Jan 1, 1973

By F. E. Wormser

"Arsenic has been recently quoted at 10 1/2 - 11 cents per pound, an increase over the prevailing price of last fall, when arsenic was cents, as against pre-war prices around 4 cents is evidently a sustained demand for this material. The following statements are intended to summarize briefly some particulars regarding arsenic.Commercial arsenic is the white arsenious oxide (As203). This is the form in which it is generally marketed, as the uses of arsenic demand its industrial application in this form. Arsenic as an element finds rather restricted use in the hardening of lead shot, ere it is to some extent replacing the use of antimony, while white arsenic is employed in a variety of manufacturing processes, chief of which is the manufacture of insecticides and sprays. The poisonous nature of most arsenic compounds, the commonly known ones being paris green and lead arsenate, and its relative cheapness, make arsenic a material suitable for this purpose. The stimulation of agriculture during the war greatly increased the demand for arsenical insecticide. Glass making consumes a large amount of arsenic as it has been impart increased brilliancy to the glass. Crude commercial arsenic is often used to kill weeds along railroad tracks. Arsenious oxide is used also for preserving hides, while the disulphide, AS2S2), finds an application in dehairing hides for the finest grade leather, as it least affects the product. Orpiment (As233), is used in textile dyeing. Wall paints and wall paper formerly consumed considerable arsenic in the form of paris green, but this use has been largely,given up due to the poisonous nature of the dust from the product. Sodium arsenate for a similar reason, is now little used in printing where it had found application. The great objection to arsenical yes and paints is the risk of poisoning from use of the manufactured article. Sodium arsenite is the principal poisonous in¬gredient of many cattle and sheep dips. Arsenic compounds are also used medicinally."

Jan 1, 1920

By John R. Reeves

The principal oil aha.le in Indiana ie known as the New Al'baZ\Y shale. It 11 ot Devonian age, and ia usually correlated with, although not certainly of the 1ame age a,, the Antrim shale of northern Michiga.. the Ohio shale of Ohio, and the Chattanooga shale ot Kentucky. Termescae • a."ld Georgia. The nqtmal thickneaa of the formation 1n Indiana 1e a.bout 100 faet, ltbough 1n the region of its out­ cro;l the tall th!::kne,111 io rarely exposed because cf the general d:.p of the strata, which is 11 about 30 feet per mile to the west. With the exception ot thin local pha1e1, the entire thickness is oil shale,

Aug 1, 1922

By Robert F. Holub

The Bureau of Mines has evaluated four working-level radiation-measurement methods and optimized them from the inherent and statistical error standpoint. Optimization with respect to various sampling and measurement times yielded no marked improvement over the existing methods. However, when appropriate approximations are made and evaluated, these two-measurement methods can be reduced to simple formulas, eliminating a need to use nomograms. These modified methods also have improved inherent error characteristics. A new method--a combination of two existing methods--with improved inherent and statistical errors has been suggested. All the pertinent equations are given, together with the computer program, so that any two-measurement method can be readily evaluated.

Jan 1, 1980

By Ryron O. Pickard

"The Berkeley safety station of the U. S. Bureau of Mines was recently informed that a geologist was killed through entering an abandoned 90-foot exploratory shaft without making a preliminary test of the shaft atmosphere. He was one of a geological party which was investigating coal lands in California. The only light that he carried into the shaft was an electric flash light. After he had descended a short distance, one of his associates on the surface heard him exclaim, ""This place is full of gas, I am coming up"", He had no more than finished the remark when he was seen to lose his grip on the ladder and fall down the shaft. He was apparently weakened from impure air in the shaft, and could no longer suppers himself on the ladder. When his body was recovered, it was found that his neck had been broken by the fall.This is the ;second recent accident that has been called to the writer's attention where a life has been lost in abandoned exploratory workings; the other fatality being in Minnesota where a mining engineer was overcome while sampling a shallow pit in iron-ore formations. This engineer was on the bottom of the shallow pit and died before help could reach him. It was generally supposed that each of these deaths were due to poisonous gases, but the writer believes, after reviewing the information available, that death in both instances may have been directly caused by deficiency in oxygen.Frequently in the daily press there are items relative to lives being lost through cleaning out old wells, and as a general rule such accidents are also caused by impure atmospheres.In every abandoned shaft or pit, or in fact, any old working where the air has been stagnant for a considerable time, there is always danger of oxygen deficiency, as the oxygen which originally comprised one-fifth of the atmosphere may be appreciably replaced by gases that do not support life, usually by carbon dioxide (black damp) or methane (fire damp). Much valuable information on the subject of mine atmospheres, mine gases, and the effect of oxygen deficiency onthe human system is contained in Miners' Circular 14, ""Gases Found in Coal Mines"" by G.F. Burrell and F. M. Seibert; Technical Paper 122, ""Effects of Oxygen Deficincy on Small Animals and Men"", by G. A. Burrell and G. G. Oberfell; and ''Chit of Properties of Mine Gees"" by G. A. Burrell. These publications of the Bureau of Mines should be rent by all who are interested in the subject of this paper."

Nov 1, 1921

By Robert S. Sanford

The Broughton and Ring magnetite deposits in Essex County, N. Y., are thought to be in a mineralized belt that extends from the Lake Sanford mining district to Lake Champlain and passes through the Mineville district. The country rook is syenite-gneiss, similar to that in the Mineville area. The Bureau of Mines conducted a vertical magnetometer survey of the area. On the Broughton properties there are no indications, magnetically, of magnetite in any degree of concentration. 'On the ding properties a number of sharp, localized magnetic anomalies were observed. One core-drill hole was bored to a, depth of 48.3 feet, also, to test one of the strongest anomalies. The field work was in progress from December 1943 to July 1944. Although no iron ore was intersected by' the drill hole, it showed that the amphibolite rook, at a depth of 6 to 100 feet below the surface, contained enough magnetite and iron sulfides to account for the anomalies recorded by the magnetometer survey.

Jan 1, 1949

By Byron. O. Pickard

"An Arizona mining company recently demonstrated a new use for the geophone by successfully locating leaks in compressed-air lines which were buried under from 1-1/2 to 2-1/2 feet of fine rock fill. The following information is abstracted from data sent to the Bureau of Mines by the superintendent of the mine.The company has two parallel main compressed-air lines, about one and one-half miles long, buried under an average of two feet of fine rock fill. One of the lines is a four-inch high-pressure (1000 pounds) main, and the other line a ten-inch low pressure (90 pounds) main. The two mains are separated by a 10 to 3C foot interval, and run from the compressor plant to the No. 2 shaft, where they are taken underground. A pump line is also buried in the immediate vicinity.Several leaks, not audible enough to be located, were developed in the Alms. Various =Abode were suggested that did not seem practicable to the management. Finally, the company obtained a Bureau of Mines' geophone and experimented with it. The first test& were conducted while the pumps were running, and while there was noise on the concrete pavement, all of Which caused so much vibration in the geophone that it was impossible to detect the leaks.The second attempt was made at the close of the day shift when the compressors were not operating and when the pumps could be shut down. The valves in the far end of the surface lines were closed, and the lines were pumped to full pressure (90 and 1,000 pounds respectively).Geophone readings were then taken at 7 or 8 foot intervals directly see the lines. Several leaks some very large in the high-pressure line were audible with the geophone at distances varying from 15 to 30 feet. A con-le leak in the low-pressure line was found at a distance of 10 feet. detecting the leaks, no difficulty was found in locating them exactly. ""At this time"", laid the superintendent of the mine, are unable to large a leak must be in order to be located under two feet of loose geophone. The fill we worked over was quite loose and dry and unfavorable for the work in question. However, we have no that the instrument is a valuable adjunct to our business""."

Aug 1, 1922

By Larmon J. Heath

The Bureau of Mines explored the use of air or natural gas for recovering additional oil from natural water-drive reservoirs with little or no original gas saturation. Engineering and production data for a field in the Mid-Continent area, results of limited laboratory experiments, and data shown in the literature were considered. Injecting air into a water-coned well could seriously damage the well. Injecting natural gas into the watered-out zone could improve the producing water-oil ratio. In the reservoir studied, oil recovery can be increased by injecting natural gas to decrease water-oil ratios. Laboratory tests on a core from the field show an increase in oil recovery of 7.6 percent of the original oil in place as a result of increasing the free gas saturation. This recovery could amount to as much as an additional 6 million barrels of oil from this field.

Jan 1, 1966

By D. W. Frommer

Processing of nonmagnetic taconites by selective flocculation-des liming and anionic flotation of calcium-activated silica has been investigated using innovative procedures as a means of improving operating costs. Areas chosen for cost-cutting evaluation were grinding, fine screening, and water conservation and reclamation. The investigation was performed on Marquette range nonmagnetic taconites in the Bureau of Mines Twin Cities Metallurgy Research Center flotation pilot plant, at feed rates ranging between 900 and 1,100 lb/hr. With fully integrated water reclamation, flotation concentrates containing 65.8 percent Fe and 3.4 percent Si02 were made in the best operating period, with accompanying weight and iron recoveries of 40 and 74 percent, respectively. During this same period, the estimated cost of both flotation and water reclamation reagents was only $0.80 per ton of concentrate (1968 pricing basis). This cost is about 15 percent lower than obtained previously at this Center for flotation reagents only. The investigation demonstrated additional potential savings in operating costs of approximately $0.96 per ton of concentrate through autogenous grinding as a substitute for ballmilling. Fine screening of intermediate concentrates was employed advantageously, but the frequency of blinding may be a factor militating against use of the screen in this flotation system.

Jan 1, 1973

By Seth C. Schaefer

The Bureau of Mines studied the standard free energies2 of formation of ferrous fluoride (FeF2) and ferric fluoride (FeF3) with high-temperature galvanic cells employing calcium fluoride doped with 1 mole-pet yttrium fluoride as the electrolyte. A free-energy change for the cell reaction Fe (s) + NiF2 (S) = FeF2 (S) + Ni (s) together with the standard free energy of formation of nickel fluoride yields a standard free energy of formation of ferrous fluoride from the elements of ?GF° = -168.74 + 30.63 x 10-3T ± 0.41 kcal/mole (897-1,098 K). (2) Combining the standard free energies of formation of magnesium fluoride, ferric oxide, and magnesium oxide with the free-energy change for the overall cell reaction 3MgO(s) + 2FeF3 (s) = 3MgF2 (S) + Fe2 03 (S) (3) gives a standard free energy of formation of ferric fluoride from the elements as ?Gf° = -281.45 + 90.46 X 10-3T ± 0,67 kcal/mole (880-935 K). (4) Free energies and equilibrium fluorine pressures for the two univariant equilibria of the Fe-F system are tabulated and illustrated.

Jan 1, 1975

By Donald P. Tuchman, Robert P. Vinson, Steven J. Page, Linda J. McWilliams, Gerald J. Joy, Steven E. Mischler, Jon C. Volkwein

The United States National Institute for Occupational Safety and Health, through an informal partnership with industry, labor, and the United States Mine Safety and Health Administration, has developed and tested a new instrument known as the Personal Dust Monitor (PDM). The new dust monitor is an integral part of the cap lamp that coal miners normally carry to work and provides continuous information about the concentration of respirable coal mine dust within the breathing zone of that individual. Previous laboratory testing demonstrated that there is a 95% con?dence that greater than 95% of individual PDM measurements fall within ±25% of reference measurements. The work presented in this paper focuses on the relationship between the PDM and respirable dust concentrations currently measured by a coal mine dust personal sampler unit utilizing a 10 mm Dorr Oliver nylon cyclone. The United Kingdom Mining Research Establishment instrument, used as the basis for coal mine respirable dust standards, had been designed speci?cally to match the United Kingdom British Medical Research Council (BMRC) criterion. The personal sampler is used with a 1.38 multiplier to convert readings to the BMRC criterion. A strati?ed random sampling design incorporating a proportionate allocation strategy was used to select a sample of mechanized mining units representative of all US underground coal mines. A sample of 180 mechanized mining units was chosen, representing approximately 20% of the mechanized mining units in production at the time the sample was selected. A total of 129 valid PDM/personal sampler dust sample sets were obtained. A weighted linear regression analysis of this data base shows that, in comparison with the personal sampler, the PDM requires a mass equivalency conversion multiplier of 1.05 [95% C.I. (1.03, 1.08)] when the small intercept term is removed from the analysis. Removal of the intercept term results in a personal sampler equivalent concentration increase of 2.9% at a PDM measurement of 2.0 mg m-3.

By L. W. Brandt

Studies of liquid-vapor equilibria of gas mixtures at the Bureau of Mines Amarillo Helium Plant Laboratory require that the equilibrium cell be maintained uniformly at any desired temperature from approximately room temperature to -320° F. The temperature-control system must also tolerate a substantial heat input, in the form of warm gas flowing into the cell, without loss of control. Temperatures between the sublimation temperature of carbon dioxide (-109° F.) and the boiling point of liquid nitrogen (320° F.) are usually obtained by methods utilizing a hydrocarbon liquid bath cooled by liquid nitrogen.4/5/6/7/ Owing to the fire hazard attending such baths, a safer method of temperature control was desirable.

Jan 1, 1955

By A. J. Barry

Two methods of testing anchorages of headed expansion-type mine roof bolts were investigated by the Federal Bureau of Mines to determine whether they are acceptable for general use. In both methods a load was applied to a tightened bolt with a hydraulic jack. A total of 80 tests, 40 using each method, was conducted in 4 test areas in 3 commercial coal mines. Electric resistance strain gages were used in all tests to indicate true bolt load as the bolt was tightened and when an additional load was applied with the hydraulic jack. The results indicate that: 1. The relationship between the true bolt load and the jack load will vary according to the test method until the two loads become equal. The bolt load-jack load relationship below this point can be determined only with strain gages. 2. The point at which the two loads become equal can be estimated by noting the load when the hardened washer becomes loose in one method, and when the bearing plate becomes loose from the roof in the other. It also can be approximated for either method of testing by noting the load when the first abrupt change in the slope of the jack load-bolt displacement curve occurs. 3. The anchorage characteristics of the test bolt can be determined from the data obtained from either method by examining the slope of the jack load-bolt displacement curve above the point at which the true bolt load is equal to the jack load.

Jan 1, 1960

Reduce face methane concentrations and help prevent miners from working under unsupported roof while moving the curtain. Approach Use water sprays on the continuous mining machine to move and direct air to the face, and also provide appropriate installation guidelines for the most effective use of the system. I n laboratory tests and under actual mining conditions, the spray fan has proved an effective means of ventilation. Recent observations in the field, however, have shown that not enough technical information is widely available on proper installation of the spray fan. This Technology News is designed to rectify that situation by providing a concise guide for spray fan installation. How the System Works Good ventilation is essential for methane control. The spray fan system repositions regular water sprays to help the normal exhaust face ventilation do a more effective job. The various water sprays help in many ways: 1. Side sprays bring clean air along the side of the miner into the off-curtain corner.

Jan 1, 1983

By J. J. Forbes

The Act of Congress authorizing the establishment of the United States Bureau of Mines became effective July 1, 1910. One of the most important duties Congress delegated to the Bureau in this Act was the study of health and safety conditions in the mineral industries. Early investigations of these conditions revealed the need of prompt Care of injured employees, as, under the difficulties inherent in their occupation, seemingly unimportant wounds if not treated promptly might become infected and cause permanent disability or even death. Many seriously injured persons brought out of mines and plants by fellow workers ignorant of approved methods of giving first aid or of safe means of transportation have been caused suffering and have had injuries accentuated to such a degree as to bring about permanent disability or death. The need of general instruction in first aid thus became apparent. BRIEF HISTORY OF FIRST-AID WORK The education of miners in the United States in first aid probably was conducted systematically first by the anthracite industry in Pennsylvania. Subsequently, first-aid training was carried on actively in Alabama and Indiana. Meanwhile, a general training campaign was carried on among industrial workers by the American Red Cross Society; when the Bureau of Mines began its nation-wide campaign of instructing employees of the mineral industries, the Red Cross turned over that part of its industrial training work to the Bureau but continued to cooperate by furnishing surgeons as judges in important first-aid contests and demonstr tions and assisted for a number of years in determining training methods.

Jan 1, 1937

By Harold W. Lipper

Helium production and shipment reached new peaks in 1.361. The Bureau of Mines produced 727 million cubic feet, 13 percent more than the 1960 record, and shipped 552 million cubic feet, 16 percent more than the 1960 record. Production exceeded demand for the third successive year, enabling the Bureau of Mines to add 174 million cubic feet to the volume stored underground for future use. At the end of 1961, the total volume of helium in underground storage in the Clifside gasfield was 44-7 million cubic feet. Contracts to implement a long-range helium conservation program were completed with four companies, obligating all the contracting authority of $47.5 million a year made available by the Congress. The four companies will finance, build, and operate helium extraction plants to recover from natural gas an annual average of 3.1 billion cubic feet of helium that would ordinarily be wasted when the helium-bearing natural gas is used for fuel.

Jan 1, 1962

"In sum, significant benefits to mine worker health have resulted and should continue to result from the synergistic scientific, engineering, and medical research in respirable dust. As always, an important objective is the rapid dissemination of the results of this research to industry. Video tapes and the Technology News series published by the United States Bureau of Mines (USBM) have been a very effective means of technology transfer. Individual no-cost subscriptions to the Technological News series are available from the Bureau of Mines, Branch of Technology Transfer, 2401 E St., N.W. Washington, DC, 20241.Two respirable dust conferences have been jointly sponsored by the Center, USBM, NIOSH, and MSHA. The proceedings from these conferences are titled:1. Coal Mine Dust Conference held in Morgantown, West Virginia on October 8-10, 1984 and2. Respirable Dust in the Mineral Industries: Health Effects, Characterization and Control held in University Park, Pa. on October 14-16, 1986.Copies of the proceedings from the first conference are available from the National Technical Information Service (NTIS):National Technical Information Service5285 Port Royal RoadSpringfield, VA 22161NTIS # PB86-169380Telephone Number: (703)487-4650Copies of the second conference proceedings, ISBN: 0-936712-76-7 (publication #3010), are available from ACGIH by writing toWilliam D. KelleyExecutive Director, A.C.G.I.H.6500 Glenway Avenue, Building D-FCincinnati, Ohio 45211Telephone Number: (513)661-7881During the period 1984-1987 more than sixty-four (64) papers and publications have been produced by the Center researchers. These publications have appeared in scientific journals and have been presented at national and international symposiums and meetings. A list of these papers, organized by topical headings, has been included in this publication.The generic center maintains a reference center that serves as a clearinghouse for technical information for the generic area and supplies reports on generic center accomplishments."

Nov 1, 1988

By L. C. IlsLey

One of the details which the engineers observe closely in conducting tests on explosion-proof type equipment at the Pittsburgh experiment Station of the Bureau of Mines is the construction of covers and their means of fastening. These covers are of particular concern from a safety standpoint, because they are very liable to be frequently disturbed for the purpose of making inspection or repairs to internal parts of motors, controllers, etc. When depended upon to permit frequent inspections it is desirable from a practical operating standpoint that the cover be easily and quickly removed and replaced. The position that been taken by the Bureau of Mines is that the questio of ease with which covers will permit inspection should be determined by the mamu- facturer and the user. If the scheme used requires considerable effort, as in the removal of several bolts, it has been assumed that the one going to all this trouble to get the covers off could be trusted to put the cover back in place properly, especially as the approval plate generally calls specific attention to this point. Where a cover is simply screwed in place, fastened by a single bolt, or secured by a hing in or other similar arrangement, the bureau has not been willing to assume that the, cover would in all cases be properly fastened and has required further security by means of a lock or seal. In the course of its investigations the bureau has had occasion to inspect and test five types of covers as follows: (1) Covers having two plane surfaces bolted together. (2) Covers having two plane surfaces held together by lugs, clamps, or other means besides bolts. (3) Covers which have screw thread and fit a similar thread in the compartment. (4). Covers having plane surfaces with the addition of a tongue-and-groove joint at about the center of the plane surface and in general secured by hinge pins or similar means of fastening. (5) Covers in the form of an annular ring accurately fitted to a motor shell which can be rotated around the shell, and thus make easy access to inspection openings in the shell and rotated back again to effectively seal these openings. Tests. have also been made of large bolted covers having a smaller and more accessible cover within the large one, which could be opened for inspection purposes.

May 1, 1930

By N. S. Dalal, X. Shi, V. Vallyathan

"INTRODUCTIONThis paper summarizes our recent investigations of the potential role of silicon-oxygen based free radical species in the biochemical mechanism of the lung injury caused by the inhalation of freshly fractured silica. The impetus for this study came from the following observations. Inhalation of crystalline silica is associated with the development of acute and chronic silicosis. Chronic silicosis is characterized by the development of sharply marked concentric fibrotic nodules in the lung. This occurs over a period of several decades with the development of progressive respiratory impairment. In contrast, acute silicosis is manifested by the filling of the alveoli with an amorphous lipoproteinaceous exudate within a short period after exposure, and rapid development of respiratory disability often leading to fatality. Most studies on the pathogenesis of silicosis have focused on the elucidation of cellular mechanisms of cell injury and the development of chronic silicosis. Because pulmonary responses to crystalline silica are distinctly different for · the acute and chronic silicosis, we hypothesized that the acute response is associated with some unique properties of ·freshly formed silica particle surfaces, due to the formation of some reactive chemical species caused by the rupturing or the silicon-oxygen bonds. In occupations involving drilling, tunneling and sandblasting operations acute silicosis can be correlated with this unique surface reactivity of silica. The formation or reactive species was demonstrated, using electron spin resonance (ESR) spectroscopy (Dalal et al., 1986; Fuibini et al., 1987; Hochstrasser and Antononi, 1972). Grinding of quartz crystals under ultra high vacuum (10-10 torr) leads to the formation of Si- and SiO• radicals and these radicals are quenched by exposure to air and other gases (Hochstrasser and Antonini, 1972). ESR signals have also been reported from micronized quartz samples that were evacuated at 413 K (Bolls et al., 1983). In order to find a more direct relationship of such radicals to acute silicosis, we made ESR measurements on quartz dusts freshly ground in ambient air and found- that indeed grinding under ambient environment leads to the formation of the Si• and SiO• type of radicals which decay on storing the dusts in air or in biological buffers (Dalai et al., 1986; Vallyathan et al., 1988). The Si• and SiO radical formation on grinding has also been reported by Fubini et al. (1987). In addition, we recently found that •OH radicals are generated in aqueous suspensions of freshly ground quartz (Dalai et al., 1988; Shi et al., 1988; Vallyathan et al., 1988). We have subsequently carried out detailed cytotoxicity and biological assays on freshly made as well as aged quartz dusts. As summarized below, the results provide new insight into the biochemical mechanism of acute silicosis."

Jan 1, 1988