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By H. Sarvamangala

Selective separation of alumina, silica, calcite and apatite from hematite was achieved through microbiologically induced flotation and flocculation in presence of bacteria (B.subtilis) and that of hematite from apatite after interaction with yeast (Saccharomyces cerevisiae). Bacterial and yeast metabolites containing extracellular proteins were characterized from mineral-grown cell free extract. Bacteria and yeast can adhere to mineral surfaces and influence subsequent separation of the minerals. Bacteria functioned as a stronger depressant for hematite, while yeast cells depressed apatite at neutral pH. Selective affinity of the bacterial and yeast cells towards the mineral surface was observed through adsorption studies. Extracellular proteins (EP) were isolated from B.subtilis and yeast. The protein profile of the EP of bacterial and yeast cells grown in presence and absence of minerals was studied. This study has demonstrated the utility and amenability of microbially-induced mineral beneficiation of iron ores through bacterial and yeast cells and their metabolic by products.

Sep 1, 2012

By K. A. Natarajan

Interaction with microorganisms results in significant surface chemical changes on minerals. For example, chemolithotrophs such as Thiobacillus ferrooxidans and Thiobacillus thiooxidans significantly alter the surface chemistry of several sulfide minerals such as pyrite, chalcopyrite, sphalerite and galena. Heterotrophic bacteria such as Bacillus sp. could alter the electrokinetic properties of oxide minerals such as hematite, alumina, silica, kaolinite and calcite. Microbe-mineral interactions could thus be beneficially utilised in mineral beneficiation as different from bioleaching. Microbially induced mineral beneficiation unlike bioleaching deals with interfacial phenomena at mineral-solution-bacteria interfaces and surface chemical changes are brought about at very rapid rates amounting to a few minutes resulting in mineral separations in an aqueous medium.

Jan 1, 2003

By K. A. Natarajan, M. N. Chandraprabha

Selective separation of minerals from complex sulphide ores is a challenging task and it is often difficult to get commercially acceptable individual concentrates by conventional selective flotation using inorganic collector reagents. Microbially-induced mineral beneficiation has proved to be more effective, economically viable and an environmentally benign process for the effective separation of various minerals. Applications of microbially-induced mineral beneficiation are demonstrated in this paper with respect to beneficiation of complex multimetal sulfides. The role of adapted microorganisms and bioreagents in the beneficiation of complex sulphide minerals are also illustrated.

Jan 1, 2009

By M. N. Chandraprabha

This paper discusses the role of the mineral-adapted acidiphilic microorganism Acidithiobacillus ferrooxidans in the beneficiation of arsenopyrite-containing multisulfides (pyrite and chalcopyrite) and the bioremediation of the resulting arsenical waste water. It was found that adaptation to minerals alters the surface properties of the microorganism. Bacterial adaptation to arsenopyrite and controlled bacterial adhesion to mineral surfaces lead to selectivity in arsenopyrite separation. Bioremoval of arsenic ions (both arsenite and arsenate ions) by Acidithiobacillus ferrooxidans is also discussed. Key words: Acidithiobacillus ferrooxidans, Bioremediation, Arsenopyrite beneficiation

Jan 1, 2009

By K. A. Natarajan

The utility of yeast, Saccharomyces cerevisiae, in the separation of quartz from hematite is demonstrated. Yeast cells, as well as their metabolites, functioned as flotation collectors, depressants or flocculants and dispersants for hematite and quartz. Interaction between yeast and the above minerals resulted in significant surface chemical changes, rendering quartz surfaces hydrophobic and hematite hydrophilic. Mineral-specific extracellular proteins and exopolysaccharides were secreted by yeast cells when grown in the presence of quartz and hematite, respectively. Quartz could be efficiently separated from hematite through microbially induced flotation and selective flocculation.

May 1, 2012

By M. N. Chandraprabha

This paper discusses the role of mineral-adapted acidiphilic microorganism Acidithiobacillus ferrooxidans on the beneficiation of arsenopyrite containing multisulfides (pyrite and chalcopyrite). Adaptation to minerals altered the surface properties of the microorganism. Bacterial adaptation to arsenopyrite and controlled bacterial adhesion to mineral surfaces lead to selectivity in arsenopyrite separation. Bioremoval of arsenic ions (both arsenite and arsenate ions) by Acidithiobacillus ferrooxidans is also discussed.

Jan 1, 2009

By A. Bruynesteyn

I would like to talk to you today about microbiological leaching or, the solubilization of metal values due to the action of Thiobacillus ferrooxidans. Bacterial attack of sulfide minerals has been going on for centuries but only recently was it discovered that the acid and iron often found in coal mine drainage is produced by bacterial action on the iron sulfides in the coals. Not until 1950 was it proven that bacteria were playing a role in the dump leaching of sulfide ores. B. C. Research became involved in microbiological leaching in 1955 while examining mine drainage from the copper-zinc mine at Britannia Beach, north of Vancouver. Since 1955, B.C. Research has maintained a continuous re¬search program on microbiological leaching which, due to its applied nature, was put under industrial sponsorship in 1962. Most of the information pro¬vided in my talk today has been gathered under this sponsored research program and I would like to express our appreciation to the many sponsors who have made our work possible.

Jan 1, 1970

By Sudhir C. Dhara

An attempt has been made on the concentration of Platinum Group Metals (PGM) from the converter matte material by microbial .leaching. Biological oxidation of base metal sulfides such as iron, copper, nickel and free sulfur present in the matte material using Thiobacillus ferrooxidans is an alternative method to concentrate the PGM-and gold. The method which produces a platinum group metals concentrate is comparable to chemical processes and electrochemical process.

Jan 1, 1995

By O. H. Tuovinen, T. M. Bhatti

Microbiological leaching has been used as an alternative approach to conventional hydrometallurgical methods of uranium extraction. In the microbiological leaching process, iron-oxidizing bacteria oxidize pyritic phases to ferric iron and sulfuric acid, and uranium is solubilized from the ore due to sulfuric acid attack. If uranium in the ore material is in the reduced, tetravalent form (UIV), a red ox reaction is involved whereby uranium is oxidized to the hexavalent form (UvI) upon dissolution. In acid-leaching systems, the primary oxidant is ferric iron, which is reduced to ferrous iron by its chemical reaction with UIV. The ferrous iron thus formed is reoxidized to ferric iron by iron-oxidizing bacteria such as Thiobacillus ferrooxidans and Leptospirillum ferrooxidans. Nutritional requirements and responses to environmental extremes of acidophilic iron-oxidizing bacteria are appraised. The S-entity in Fe-sulfides is oxidized to sulfate by bacteria similar to Thiobacillus ferrooxidans and Thiobacillus thiooxidans. Pyrite and marcasite oxidation is a sulfuric acid forming reaction. Heap, dump and in situ leach techniques are feasible as bacterial leaching systems.

Jan 1, 2000

By Lawson E, Purkiss S. A R, Van Ashwegen p C

It is only in the past decade or so that industrial exploitation of biological resources and techniques has become regarded as a distinct discipline. Commonly referred to as Biotechnology, it embraces biochemistry, microbiology and the engineering sciences and is mainly aimed at the biological conversion of suitable raw materials into desired end products. The catalytic agents are more often than not bacteria although other micro-organisms such as fungi are used. The range of micro-organisms used is wide and the choice depends upon the desired end product, which in turn depends upon the metabolic attributes, genetically engineered or otherwise, of the cells. The metabolic diversity of micro-organisms has been applied to processes such as antibiotic production, food processing, brewing and chemical production, and of course extraction of metals from ores. Metabolic diversity is, to a certain extent, based on nutrition, which, in its simplest form, can be divided into energy source, carbon requirements and nature of the electron donor producing reducing power to the cells. The source of energy may be light, in which case the organisms are phototrophic (eg green plants, algae). The carbon requirements and reducing power may come from organic sources. These organisms are then called heterotrophic (eg man and most bacteria, including the well known Escherichia colt). However, there is a group of bacteria whose energy metabolism depends upon chemical redox reactions, use inorganic carbon (CO2) and obtain their reducing power from reduced chemicals such as metal sulphides. These are known as chemolithotrophs. They play an important role in mineral recycling in nature and are also ideal for extraction of metals from ores. This is because they are extremeophiles being acidophilic (grow at very low pH) and sometimes also thermophilic (grow at very high temperatures). These bacteria operate in such hostile environments that competition, or contamination, by other organisms does not occur. Extraction of metals from ores by chemolithotrophic bacteria is commonly referred to as biohydrometallurgy. Other related areas are bioadsorption of metals from dilute solution,- microbial treatment of metal contaminated wastes and removal of toxic chemicals produced or used during mineral processing. This paper basically reviews the work that Gencor has undertaken in the field of biohydrometallurgy and the advancements it has made in commercialising the technology, but also touches on certain long-term areas of research that are currently under investigation.

Jan 1, 1995

By J J. Plumb, P D. Franzmann

A study was conducted to describe the microbiology of the MICCL Monywa chalcocite heap bioleaching operation. Microorganisms were detected in the heap using culture-based techniques and cultureindependent PCR-DGGE analysis of the 16S rRNA gene. The metabolic requirements, temperature and pH optima were determined for selected strains isolated from the heap material. Six strains (BH1-BH6) were enriched and isolated from heap solids and leachate samples. Phylogenetic analysis of the 16S rRNA genes showed that strains BH3 and BH4 were closely related to the acidophilic bacterium Acidithiobacillus caldus and strains BH5 and BH6 were closely related to Leptospirillum ferriphilum. The optimum growth temperature for L. ferriphilum was 41.3¦C and the optimum pH range was 1.1 to 1.5. A new species of Archaea was isolated from the heap samples. Cells of the proposed species, Ferroplasma cyprexacervatum (æcyprusÆ L.n. meaning copper; æexacervoÆ L.v. meaning æto heap upÆ), are non-motile pleomorphic cocci, capable of chemomixotrophic growth with ferrous sulfate and yeast extract. Cells grow anaerobically on ferric iron in the presence of potassium tetrathionate and yeast extract as electron donors. Growth occurs from 14¦C to 63¦C, with an optimum temperature of 55.2¦C. The optimal pH for growth was 1.0 - 1.2. The species was phylogenetically most closely related to Ferroplasma acidiphilum with a 16S rRNA gene sequence similarity of 95 per cent. Molecular analysis of mine samples (16S rRNA PCR-DGGE) detected the above-mentioned cultured strains of microorganisms as well as species of Sulfobacillus and other unknown Proteobacteria. The microbial community of the Myanmar bioleach heaps contained similar levels of diversity to a dump bioleach operation in Chile, but contained greater amounts of biomass than another heap bioleach operation in Australia. The operating parameters of the Myanmar heaps (low pH and moderate temperature) have selected for the growth of moderately thermophilic microorganisms that are more commonly found in extremely low pH acidic mine drainage sites than in heap systems. The novel species of Ferroplasma is thought to be involved in the aerobic and anaerobic cycling of iron within the heap bioleaching environment.

Jan 1, 2004

By Roe-Hoan Yoon

"Industrial flotation columns often suffer from low recoveries and high maintenance requirements because of poorly-designed air spargers. The MicrocelTM bubble generators were developed at Virginia Tech to overcome these problems. In this system, small air bubbles are generated by circulating a portion of the flotation pulp through a set of static in-line mixers. The small bubbles improve flotation kinetics and allow a higher recovery (or capacity) to be achieved. Other advantages of the system include reduced air consumption, plug-free operation and on-line maintenance. This article discusses the advantages of the MicrocelTM bubble generators and presents examples of successful in-plant demonstrations.IntroductionColumn cells are widely accepted in industry for cleaner operations because they are more selective than mechanically-agitated conventional cells. The improved selectivity can be attributed to the use of wash water which minimizes the entrainment of fine gangue particles into the froth product. Columns may also produce higher recoveries if the air sparging system is properly designed and steps are taken to minimize back-mixing. Furthermore, the quiescent conditions afforded by column cells may be beneficial for recovering coarse particles that are readily detached from bubbles under more turbulent conditions. Also, properly-designed columns offer advantages in terms of savings in floor space, reduced operating and capital costs, and simplified circuitry.It is unfortunate, however, that some of the advantages of columns are not fully realized in industrial practice. Some column circuits are unable to achieve their design capacity, while others require extensive maintenance programs. These shortcomings can usually be attributed to deficiencies associated with the design, scale-up and operation of the air-sparging system. In this article, some of the basic problems associated with commercial air sparging systems are discussed and alternatives are suggested."

Jan 1, 1994

By M. T. Styles, E. Condliffe, R. C. Leake, R. J. Chapman, D. J. Bland

Paper presented at Mineralisation in the Caledonides, the Mike Gallagher memorial meeting held in Edinburgh, 27-28 June 1996. Quantitative point analysis and microchemical mapping identified 52 different types of mineral in inclusions, usually ranging in size from 5 to less than 1 micrometre, inherited from source bedrock. A combination of grain chemistry, heterogeneity and inclusions provides a grain signature reflecting the type and history of source mineralisation. Several grains from an alluvial site are needed, to allow for bedrock variation and the presence of inclusions in only 10-20% of grains. A total of 1 530 grains obtained from 30 localities in Scotland were of ten types, two of them minor variants. Two types in the Highlands may be related to centres of Caledonian igneous activity, but the most widespread is likely to have been of deep-seated origin and structurally controlled. Gold in the Ochil Hills is probably from a source associated with Devonian andesitic activity. In the Southern Uplands, grain types reflect two separate episodes of mesothermal lode gold mineralisation and relationships with Permian red beds/mafic volcanics and with Tertiary dykes

Jun 19, 1905

By Q. F. Wang

Spontaneous combustion hazard of loose coal in the top-coal caving region of entry affects the safety of the entry and top- caving longwall face seriously. In this paper, by combining on- site temperature tests with lab tests and numerical simulation, the characteristics of microcirculation close to the top-coal caving region are analyzed. A model for the temperature fields of the microcirculation in loose coal is presented. Based on this model, the temperature distribution and possible self-ignition region can be predicted. Further, the heat and mass transfers in microcirculation are studied. Finally, the mechanism of oxygenation during the spontaneous combustion of microcirculation in loose coal of the top-coal caving region of entries is put forward. The research results in this papa may provide ideas for the prevention and putting out of the fire caused by spontaneous combustion in the caving zone.

Jan 1, 2004

By Scott G. Britton

Microcomputeranalysis has long been a technique for engineering professionals to improve the theoretical and practical design of continuous mining systems. Such work has been typically composed of modeling observed events and then introducing a variety of known elements with predicted results to compare against the results of the model. Programs written in BASIC, Cobol, Fortran or other language dominated such efforts. These programs have taken years to develop and prove out. This article explores a new management tool used daily at Tanoma Mining Co. (TMC) to track and improve continuous mining systems through the use of microcomputers and Lotus l-2-3 software. Data provided by section foreman, shift foreman, and maintenance personnel are analyzed against predetermined goals. In this way, new production targets are developed, weaknesses in the current mining systems are identified, and the results of changes are measured. The current results of this system on the productive output of TMC systems is on the order of 10% improvement. Thin seam coal mining is an industry within the broader industry of coal mining. Thin seam mining faces the same cost pressures and engineering problems as its higher-seam competitors without the advantages of moving volume per foot of extracted material to stay at an economic parity with others requires additional creativity within the management teams to extract higher productivity gains from the existing workforce and mining systems. TMC operates a deep mine in Indiana bounty, PA, about one hour northeast of Pittsburgh.

Jan 1, 1991

By S. G. Britton

Microcomputer analysis has long been a technique for engineering professionals to improve the theoretical and practical design of continuous mining systems. Such work has been typically composed of modeling observed events and then introducing a variety of known elements with predicted results to compare against the results of the model. Programs written in BASIC, Cobol, Fortran, or other language dominated such efforts. These programs have taken years to develop and prove out. This paper explores a new management tool used at TMC on a daily basis to track and improve continuous mining systems through the use of microcomputers and Lotus 1-2-3 software. By analyzing data provided by section foreman, shift foreman, and maintenance personnel against predetermined goals, new production targets are developed, weaknesses in the current mining systems are identified, and the results of changes are measured. The results of this system on the productive output of TMC systems are on the order of ten percent improvement.

Jan 1, 1991

By D. S. DeLong, A. Giza, D. G. Fraser, V. A. Robinson, T. B. Whitmoyer, J. E. Sneckenberger

"A cotton dust animal exposure system has been modified to incorporate a microcomputer feedback loop to monitor particle concentrations. The loop's major components are a minature real-time aerosol monitor (miniram) to estimate concentrations and a microcomputer to read the miniram's data and to control a stepper motor that adjusts the amount of dilutant air mixing with respirable cotton dust. Ninety minute trials of this system indicate that the standard deviation of particle concentrations is within 0.68 Mg/m3 of the average concentration, insuring a near constant concentration of respirable dust throughout the exposure.INTRODUCTION Previous workWeyel et al. (1984) have determined that respirable cotton dust can be shaken from bulk cotton samples using acoustical energy. The particle generator designed to utilize this fact operates like a commercially available sonic sifter, but on a much larger scale.While this generator successfully resuspended trapped respirable cotton dust, the generator output concentration decreased exponentially with time. This disadvantage was solved by Frazer et al. (1986) by operating the generator with a sinusoidal voltage near the minimum resonance frequency of the generator. This adjustment enabled the generator to produce respirable cotton dust for six hours or longer.Frazer et al. (1987) utilized the modified acoustical generator to produce a cotton dust animal exposure system. The cotton dust concentrations were estimated by a miniram, a light scattering aerosol Monitor. The system operator used the miniram values to adjust the system's parameters to maintain the desired concentration level.PurposeThe objective of this study was to introduce a computerized feedback loop to the operator controlled cotton dust animal exposure system. The miniram's estimates were analyzed by a microcomputer to determine what adjustments in the flow of dilutant air were necessary to maintain the desired cotton dust concentration. These modifications were intended to improve system performance and ease the system operator's work burden."

Jan 1, 1988

By J. K. Whyatt

A microcomputer controlled data acquisition system has been developed for automatic monitoring of underground instrumentation from a remote location. The system has been used extensively by Spokane Research Center personnel for monitoring rock instrumentation in shafts, drifts, and stopes in hardrock and coal mines, especially where instrument access is limited. Ruggedness was a major consideration in designing the underground portion of the system and it has performed satisfactorily under extreme environmental conditions, including temperatures to 1100 F, 100% humidity, and falling water. The major advantage of the system is that it multiplexes the data from 16 channels through a single twisted pair cable to the receiver, significantly reducing cabling requirements. This receiving station can be as far as 8,000 ft from the instruments without the addition of line drivers. The receiver maintains a data buffer with the latest scans, which is periodical 1 y accessed by a micro- computer, either locally or through a modem, that automatically records and reduces the data. Utilities allow on-site plotting of data and uploading of data to mainframe computers.

Jan 1, 1986

By C. Dinis da Gama

In order to reduce the complexity of mechanisms influencing rock. fragmentation by blasting a simulation approach is proposed, using the capabilities of microcomputer interactive graphics. Situations involving cratering end bench blasting are simulated, and both jointed and intact rocks can be subjected to explosive action, taking into consideration its breakage mechanisms. Size analysis curves relating rock distribution before and after the blasts are determined and compared with field data.

Jan 1, 1984

By C. Dinis da Cama

In order to reduce the complexity of mechanisms influencing rock- fragmentation by blasting a simulation approach is proposed, using the capabilities of micro-computer interactive graphics. Situations involving cratering and bench blasting are simulated, and both jointed and intact rocks can be subjected to explosive action, taking into consideration its breakage mechanisms. Size analysis curves relating rock distribution before and after the blasts are determined and compared with field data.

Jan 1, 1984